Team:Rice University/Notebook/14 June 2008

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(New page: =Saturday 14 June= *Taylor Stevenson **Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were i...)
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*Taylor Stevenson
*Taylor Stevenson
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**Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator.  Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).   
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**Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator.  Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).   
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**'''Result'''-both plates showed bacterial growth at all spots after 24h.  
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**'''Result'''-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.
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[[image:20080614_30*C_VCS_lysogen]] [[image:20080614_42*C_VCS_lysogen]]
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Revision as of 20:09, 25 June 2008

Saturday 14 June

  • Taylor Stevenson
    • Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
    • Result-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.

File:20080614 30*C VCS lysogen File:20080614 42*C VCS lysogen





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