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Team:Rice University/Notebook/14 June 2008
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**Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic). | **Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic). | ||
**'''Result'''-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen. | **'''Result'''-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen. | ||
| - | [[image:20080614_30*C_VCS_lysogen.jpg|300px]] [[image:20080614_42*C_VCS_lysogen.jpg|300px]] | + | [[image:20080614_30*C_VCS_lysogen.jpg|300px]] [[image:20080614_42*C_VCS_lysogen.jpg|300px]] The upper left spot is a negative control used to gauge aseptic technique. No growth on either plate indicates that each well was isolated. The only colony that appears to have grown @ 30*C, but not at 42*C was the third from the right at the top. This colony will be further screened for presence of a lysogen. |
| - | The upper left spot is a negative control used to gauge aseptic technique. No growth on either plate indicates that each well was isolated. The only colony that appears to have grown @ 30*C, but not at 42*C was the third from the right at the top. This colony will be further screened for presence of a lysogen. | + | |
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Revision as of 20:16, 25 June 2008
Saturday 14 June
- Taylor Stevenson
- Phage infected VCS257 colonies cultured in 96 deep-well plate were spotted onto two LB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
- Result-both plates showed bacterial growth at all but one spot after 24h. The one spot appears to be a lysogen.
The upper left spot is a negative control used to gauge aseptic technique. No growth on either plate indicates that each well was isolated. The only colony that appears to have grown @ 30*C, but not at 42*C was the third from the right at the top. This colony will be further screened for presence of a lysogen.
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