Team:Chiba/Calendar-Home/20 September 2008

From 2008.igem.org

(Difference between revisions)
(→Laboratory work)
Line 15: Line 15:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>H<sub>2</sub>O</td>
+
<td>H<sub>2</sub>O(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
Line 44: Line 44:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>3</td><td>3</td>
<td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ</td>
+
<td>XbaI</td>
<td>0.25?</td><td>0.25</td>
<td>0.25?</td><td>0.25</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
-
<td>0.5?</td><td>0.5</td>
+
<td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 2</td>
+
<td>Buffer 2(&mu;L)</td>
<td>2</td><td>2</td>
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
-
<td>2?</td><td>2?</td>
+
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>?</td><td>12.5</td>
<td>?</td><td>12.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
Line 82: Line 82:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>24</td><td>24</td>
<td>24</td><td>24</td>
</tr>
</tr>

Revision as of 19:54, 29 October 2008

>Home | Notebook

19 September 2008 <|> 21 September 2008

Laboratory work

Team:Communication

--->(19/9)Mini prep

--->Gel Check

Sample Name Lagation(J04500+C0161)
Sample DNA(μL) 1
Loading Dye(μL) 1
H2O(μL) 4
TOTAL(μL) 6
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
--->Digestion Test
Sample No. 1-45-8
Sample DNA(μL) 33
XbaI 0.25?0.25
SpeI 0.50.5
Buffer 2(μL) 22
BSA(μL) 22
dH2O(μL) ?12.5
TOTAL(μL) 2020


--->Gel Check
Sample No. 1-45~8
Sample DNA(μL) 2020
Loading Dye(μL) 44
TOTAL(μL) 2424
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
  5. 　-> OK
  6. 　-> OK
  7. 　-> OK
  8. 　-> OK

--->(?/9)Transformation

--->(22/9)phenotype check


Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/20_September_2008"