Team:Chiba/Calendar-Home/26 September 2008

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(Difference between revisions)
(New page: >Home | Notebook 25 September 2008 <|[[Team:Chiba/Calendar-Home/27 September 2008|> 27 September 2008...)
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>culture</td>
+
<td>culture(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Fwd primer</td>
+
<td>Fwd primer(&mu;L)</td>
<td>1.5</td>
<td>1.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Rev primer</td>
+
<td>Rev primer(&mu;L)</td>
<td>1.5</td>
<td>1.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Taq(DNA pol)</td>
+
<td>Taq(DNA pol)(&mu;L)</td>
<td>0.2</td>
<td>0.2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dNTPmix</td>
+
<td>dNTPmix(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>thermo pol Buffer</td>
+
<td>thermo pol Buffer(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
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</table>
</table>
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<br>->95℃ 5min ->(95℃ 1min 50℃ 30sec　72℃ 1min)x25 ->72℃ 10min
+
<br>->95&deg;C 5 min ->(95&deg;C 1 min 50&deg;C 30 sec　72&deg;C 1 min)&times;25 ->72&deg;C 10 min
-
<br>->Gel cheack
+
<br>->Gel check
<br>->MINI Prep
<br>->MINI Prep

Revision as of 20:11, 29 October 2008

>Home | Notebook

25 September 2008 <|> 27 September 2008

Laboratory work

Team:Communication

Team:Output


TIME DELAY TEST


colony PCR
Ligation product(26,September)x7
Vector:BBa_R0062

Sample No. each
culture(μL) 1
Fwd primer(μL) 1.5
Rev primer(μL) 1.5
Taq(DNA pol)(μL) 0.2
dNTPmix(μL) 3
thermo pol Buffer(μL) 3
dH2O 19.8
TOTAL 30


->95°C 5 min ->(95°C 1 min 50°C 30 sec　72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/26_September_2008"