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Team:Chiba/Calendar-Home/26 September 2008
From 2008.igem.org
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<br>colony PCR | <br>colony PCR | ||
| - | <br>Ligation product(26,September) | + | <br>Ligation product(26,September)×7 |
<br>Vector:BBa_R0062 | <br>Vector:BBa_R0062 | ||
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
Revision as of 20:11, 29 October 2008
25 September 2008 <|> 27 September 2008
Laboratory work
Team:Communication
Team:Output
TIME DELAY TEST
colony PCR
Ligation product(26,September)×7
Vector:BBa_R0062
| Sample No. | each |
| culture(μL) | 1 |
| Fwd primer(μL) | 1.5 |
| Rev primer(μL) | 1.5 |
| Taq(DNA pol)(μL) | 0.2 |
| dNTPmix(μL) | 3 |
| thermo pol Buffer(μL) | 3 |
| dH2O | 19.8 |
| TOTAL | 30 |
->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep
