Team:Michigan/Project/LandingPads

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stuff about polylinker
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<font color=royalblue>Landing Pads:</font> aid in the insertion of biological constructs onto the chromosome of <i>E. coli</i>
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<font color=royalblue>Landing Pad Goals:</font>
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* BioBrick compatibility
 +
* Easy phenotypic screening
 +
* Easy sequencing
 +
* Limit noise
 +
 
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<font color=royalblue>Polylinker Features:</font>
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* <font color=orangered>BioBrick compatible restriction sites</font> - these allow for standard BioBrick construction in forward and reverse directions
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* <font color=lightseagreen>Additional building restriction sites</font> - these are for building the landing pad, as well as changing the homologous regions and drug resistance gene (can also be used for building if needed)
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* <font color=firebrick>Bar code sequence</font> - a 20 bp segment that allows for easy sequencing
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* <font color=blueviolet>Drug resistance gene</font> - this allows for easy phenotypic screening
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* <font color=deeppink>Homologous regions</font> - these specify where on the chromosome you wish to put your constructs.
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Revision as of 22:18, 29 October 2008


Michigan iGEM website header.jpg

HOME THE TEAM THE PROJECT REGISTRY PARTS NOTEBOOK


BioBrick Landing Pads

BioBrick Landing Pad Polylinker

Landing Pads: aid in the insertion of biological constructs onto the chromosome of E. coli

Landing Pad Goals:

  • BioBrick compatibility
  • Easy phenotypic screening
  • Easy sequencing
  • Limit noise

Polylinker Features:

  • BioBrick compatible restriction sites - these allow for standard BioBrick construction in forward and reverse directions
  • Additional building restriction sites - these are for building the landing pad, as well as changing the homologous regions and drug resistance gene (can also be used for building if needed)
  • Bar code sequence - a 20 bp segment that allows for easy sequencing
  • Drug resistance gene - this allows for easy phenotypic screening
  • Homologous regions - these specify where on the chromosome you wish to put your constructs.
Landing Pad Polylinker - gold.png

Landing Pads used for the Sequestilator

stuff about landing pad plasmids

Landing pad plasmid - gold.png





Learn more about Landing Pads [http://synthbio.engin.umich.edu/wiki/index.php/BioBrick_Landing_Pad Here!]