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User:University of Washington/27 June 2008
From 2008.igem.org
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== Yeast Shuttle Plasmid == | == Yeast Shuttle Plasmid == | ||
| - | - 1.5 mL 15% glycerol stocks of the overnight yeast growth cultures were made by combining 563 uL of 40% glycerol and 937 uL yeast culture, then stored at -80 degrees Celsius. | + | - 1.5 mL 15% glycerol stocks of the overnight BY4741 yeast growth cultures were made by combining 563 uL of 40% glycerol and 937 uL yeast culture, then stored at -80 degrees Celsius. |
== Conjugation == | == Conjugation == | ||
Revision as of 19:05, 27 June 2008
Yeast Shuttle Plasmid
- 1.5 mL 15% glycerol stocks of the overnight BY4741 yeast growth cultures were made by combining 563 uL of 40% glycerol and 937 uL yeast culture, then stored at -80 degrees Celsius.
Conjugation
Mini Prep
·R0042 (LuxR promoter)
Glycerol
·R0042 (LuxR promoter)
Team:University_of_Washington/Notebook#Notebook
