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18:30, 20 October 2008 (diff | hist) N Waterloo/24 August 2008 ‎ (New page: 08/08/24 Sun [x] reinoculate pJET + gene 6 [] inoculate 2 tubes each of a couple RBS-gene6 samples that looked good based on Friday's colony PCR results - Monday: make...) (top)
18:23, 20 October 2008 (diff | hist) N Waterloo/22 August 2008 ‎ (New page: 08/08/22 Fri [x] miniprep of TT, RBS, 8 samples [] prepare list for transformations ) (top)
18:21, 20 October 2008 (diff | hist) N Waterloo/21 August 2008 ‎ (New page: 08/08/21 Thurs [x] gel of full digestion digestion - not left long enough, will repeat - TT did not work, will reinoculate [] gel extraction [] ligation []...) (top)
18:07, 20 October 2008 (diff | hist) N Waterloo/20 August 2008 ‎ (New page: 08/08/20 Wed [x] miniprep of inoculatants [x] diagnostic digestion [x] diag gel of inoculants and PCR from Tues [] diagnostic gel of sketchy DNA [] gel and ...) (top)
17:59, 20 October 2008 (diff | hist) N Waterloo/19 August 2008 ‎ (New page: 08/08/19 Tues [x] inoculate good colonies from colony PCR [x] glycerol stock of iGEM stabs [x] repeat PCR phb testing - Try the ones that didn't work again, thi...) (top)
17:56, 20 October 2008 (diff | hist) N Waterloo/18 August 2008 ‎ (New page: 08/08/18 Mon [x] check gel of PHB PCR products [x] GEx of PHB PCR products (if PCR successful based on check gel) [x] colony PCR of c0051', c0052', gene 6' [x] di...) (top)
17:52, 20 October 2008 (diff | hist) N Waterloo/17 August 2008 ‎ (New page: 08/08/17 Sun [x] PCR of PHB A, B, C genes using genomic DNA and Phusion polymerase - Try it with the 1:100 dilution of Rm1021 CB (cosmid bank) DNA, left in the "good const...) (top)
17:40, 20 October 2008 (diff | hist) N Waterloo/15 August 2008 ‎ (New page: 08/08/15 Fri [x] diag gel of PCR from yesterday [x] PCR (colony PCR testing with RBS) [x] diag gel of PCR ) (top)
17:37, 20 October 2008 (diff | hist) N Waterloo/14 August 2008 ‎ (New page: 8/08/14 Thurs [x] send gene 6 for sequencing [] p/s/i of c0052', c0051', T7 gene 6 + RBS [x] miniprep of inoculatants from wed [x] Gel of overnight PCR of phbA ...) (top)
17:28, 20 October 2008 (diff | hist) N Waterloo/13 August 2008 ‎ (New page: 08/08/13 Wed [x] gel of full digestion (c0052', c0051', T7 gene6-27) [x] gel extraction [x] check gel of extraction [x] ligation of parts from extraction and c0052...) (top)
17:16, 20 October 2008 (diff | hist) N Waterloo/12 August 2008 ‎ (New page: 08/08/12 Tues [x] kit prep T7 gene 6 and gene 5 - High Priority [x] diagnostic digest of kit prep of gene 6&5 - Double (e/s) and Single (e) [] run gel of diagnost...) (top)
17:12, 20 October 2008 (diff | hist) N Waterloo/11 August 2008 ‎ (New page: 08/08/11 Mon [x] transformation of T7 gene 5 (4) in pJET [x] Inoculation of gene 5&6 colonies from pJET transformants [x] inoculate c0051' and c0052' [x] inoculate ...) (top)
17:09, 20 October 2008 (diff | hist) N Waterloo/10 August 2008 ‎ (New page: 10/08/08 Sun [] Inoculation of gene 5&6 colonies from pJET transformants - no one was able to come in for this) (top)
17:06, 20 October 2008 (diff | hist) N Waterloo/9 August 2008 ‎ (New page: 09/08/08 Sat [x] gel of PCR T7 gene 5 from friday [x] gel extraction of PCR [x] pJET ligation [x] transformation ) (top)
20:28, 17 October 2008 (diff | hist) N Waterloo/7 August 2008 ‎ (New page: 08/08/07 Thurs [] p/s/i of c0051' and c0052' - will wait until colonies are bigger [x] repeat of test PCR T7 gene 5 [x] diagnostic gel of PCR [] if colonies are b...) (top)
20:26, 17 October 2008 (diff | hist) N Waterloo/8 August 2008 ‎ (New page: 08/08/08 Fri [x] colony PCR of T7 gene 6, c0051', c0052' [x] repeat of PCR t7 gene 5 [x] diag gel of colony pcr and t7 gene 5 PCR ) (top)
18:41, 17 October 2008 (diff | hist) N Waterloo/6 August 2008 ‎ (New page: 08/08/06 Wed [x] extraction gel of PCR (gene 5) and full digest (TT, RBS, ligation controls) [x] gel extraction of above [x] ligation of RBS + C0051, RBS + C0052, lig...) (top)
18:27, 17 October 2008 (diff | hist) N Waterloo/5 August 2008 ‎ (New page: 08/08/05 Tues [x] full gel of PCR T7 gene 6 (samples 3 and 4) [x] gel extract gene 6. (x2) [x] diagnostic gel of PCR gene 6 (x2) [x] full digestion of RBS (1) TT ...) (top)
18:56, 16 October 2008 (diff | hist) N Waterloo/1 August 2008 ‎ (New page: 08/08/01 Fri [x] Repeat gene 6 PCR with iProof - iProof worked, but not the home-made PFU - positive control didn't work either [x] diagnostic gel ) (top)
18:54, 16 October 2008 (diff | hist) N Waterloo/31 July 2008 ‎ (New page: 08/07/31 Thurs [x] full gel of PCR products (gene 6) [x] diagnostic gel of t7 gene 6, t7 gene 5 (speedvacced from yesterday) - Gene 5 is ok, but Gene 6 didn't work ...) (top)
18:50, 16 October 2008 (diff | hist) N Waterloo/30 July 2008 ‎ (New page: 08/07/30 Wed [] p/s/i of POCI rfp and gfp - no growth observed - religate and retransform [x] full digestion of c0052, c0051 [x] diagnostic digest of yesterd...) (top)
18:45, 16 October 2008 (diff | hist) N Waterloo/29 July 2008 ‎ (New page: 08/07/29 Tues [x] run full gel of new PCR results [x] full gel of PCR 12 (25/7) [x] transformation of POCI, gfp and rfp [x] gel extraction [x] frozen stock ...) (top)
18:43, 16 October 2008 (diff | hist) N Waterloo/28 July 2008 ‎ (New page: 08/07/28 Mon [x] gel of PCR [x] run check gel of gel extraction product - not successful. looks like no dna - will repeat PCR [] if above successful, Clone T7...) (top)
18:39, 16 October 2008 (diff | hist) N Waterloo/25 July 2008 ‎ (New page: 08/07/25 Fri [x] autoclave waste [x] PCR testing of different conditions (glycerol, DMSO, iProof, MgSO4) [x] full gel of Gene 6 [x] gel extraction/purification o...) (top)
18:38, 16 October 2008 (diff | hist) N Waterloo/24 July 2008 ‎ (New page: 08/07/24 Thurs [x] diagnostic gel of PCR cloning T7 gene 5 [x] throw out sketchy DNA [] if cloning worked, full gel and cloning into commercial vector - Only pos...) (top)
18:36, 16 October 2008 (diff | hist) N Waterloo/23 July 2008 ‎ (New page: 8/07/23 Wed [x] decide on a reverse TT [] check details of synthesis deal [] get sequences, design, etc. to order [x] miniprep transformants - Some transfo...) (top)
18:31, 16 October 2008 (diff | hist) N Waterloo/22 July 2008 ‎ (New page: 08/07/22 Tues [x] autoclave tips/tubes [x] cloning PCR of T7 genes 5 and 6, PmeIR (colony PCR) from LB broth [x] diagnostic digest and gel of c0052'(2-8) and gene DN...) (top)
18:29, 16 October 2008 (diff | hist) N Waterloo/21 July 2008 ‎ (New page: 08/07/21 Mon [x] ligation of plac rfp'', POCI rfp'', POCI gfp'', Pconst rfp'', Pconst gfp'' [x] transformations of ligations (and plac gfp'' from previously ligated DNA)<...) (top)
18:26, 16 October 2008 (diff | hist) N Waterloo/18 July 2008 ‎ (New page: 08/07/18 Fri [x] miniprep c0052' transformant incoulations and glycerol stock inoculations [x] gel extraction and purification of plac rfp'', plac gfp'', POCI rfp'', POCI...) (top)
18:24, 16 October 2008 (diff | hist) N Waterloo/17 July 2008 ‎ (New page: 08/07/17 Thurs [x] miniprep of transformants inoculations (rfp'') [x] diagnostic digest of transformants and c0051, c0052 [x] gel of diagnostic digest [x] glyce...) (top)
18:09, 16 October 2008 (diff | hist) N Waterloo/16 July 2008 ‎ (New page: 08/07/16 Wed [] colony PCR of CI' (51) and CI' (52) [] inoculation [] test Pfu with new buffer at the same time - no growth in ligation plates of c0052 and c0051<b...) (top)
18:03, 16 October 2008 (diff | hist) N Waterloo/15 July 2008 ‎ (New page: 08/07/15 Tues [x] observe results from competent cells test, rfp'', C0052', C0051', streaked placgfp'' to UV competent cells - placgfp'' grew, [rfp'', C0052',C0051' did no...) (top)
17:54, 16 October 2008 (diff | hist) N Waterloo/14 July 2008 ‎ (New page: 08/07/14 Mon [x] transformation of ligation (rfp''), CI' (51), CI' (52) [x] competent cells test (LB and no DNA, LB and DNA, +ve and no DNA, +ve and DNA) [x] make I...) (top)
17:52, 16 October 2008 (diff | hist) N Waterloo/11 July 2008 ‎ (New page: 8/07/11 Fri [x] gel of diagnostic digest from yesterday. [x] miniprep P. mendocina, stabs from iGEM (2). [x] Make glycerol stock of P. mendocina KR1, stabs if it gr...) (top)
17:49, 16 October 2008 (diff | hist) N Waterloo/10 July 2008 ‎ (New page: 08/07/10 Thurs [x] competent cells (finish) [x] gel continued from yesterday (PCI (1-3), Pconst (1-2), PtetR (1-2), PLacOCI(1-3), TTr(2,5) and uncuts) [x] gel purifi...) (top)
17:45, 16 October 2008 (diff | hist) N Waterloo/9 July 2008 ‎ (New page: 08/07/09 Wed [x] Diagnostic digest and gel of TTr (2 and 5, uncut and single cut) + Promoters (single cut + uncut + control) [x] full digest of rfp' and tt and gel and ge...) (top)
17:42, 16 October 2008 (diff | hist) N Waterloo/8 July 2008 ‎ (New page: 08/07/08 Tues [x] autoclave SOC media [x] Make more Amp plates. There are still 12 left for Kan [x] make competent cells - finished part I [x] make glycero...) (top)
17:39, 16 October 2008 (diff | hist) N Waterloo/7 July 2008 ‎ (New page: 08/07/07 Mon [x] make SOC media and TE buffer and autoclave both [x] elute PocI and lacI from 2008 registry, follow protocol exactly. [x] transformation of 2008 regi...) (top)
17:35, 16 October 2008 (diff | hist) N Waterloo/4 July 2008 ‎ (New page: 08/07/04 Fri [] p/s/i of PocI and lacI if there is growth - no growth observed, will elute from 2008 registry [x] Mini Prep - concentrations between 2700 and 40...) (top)
17:30, 16 October 2008 (diff | hist) N Waterloo/3 July 2008 ‎ (New page: 08/07/03 Thurs p/s/i Transformation from (2/7), 3 samples - TT(reverse) good, was put in 4 degree - PocI and lacI had no growth, so retransformed PCR to test...) (top)
16:06, 16 October 2008 (diff | hist) N Waterloo/2 July 2008 ‎ (New page: 08/07/02 Wed [x] Elution - TT (reverse) B0014 1-1G - PocI R0051 - lacI [x] Transformation - Controls - above elutions (TT reverse, PocI, lacI) [x...) (top)
15:58, 16 October 2008 (diff | hist) N Waterloo/27 June 2008 ‎ (New page: 08/06/27 Friday [x] Gel of 06/26 PCR products - assess which pol.s/buffers are working will run PCR again - GEx Pfu product (if successful) to sequence) (top)
15:54, 16 October 2008 (diff | hist) N Waterloo/26 June 2008 ‎ (New page: 08/06/26 Thursday *Should have all the registration parts in by today !!* [x] Repeat Gel of 2007 Registry [x] streak - cc on LB - Placgfp'' Transformation...) (top)
15:46, 16 October 2008 (diff | hist) N Waterloo/25 June 2008 ‎ (New page: 08/06/25 Wednesday Mini prep, Partial digest and Diganostic gel [x] 2007 registry parts (~ 1.5hr) - Repeat Gel tomrrow [] 2008 registry parts - No growt...) (top)
15:40, 16 October 2008 (diff | hist) N Waterloo/24 June 2008 ‎ (New page: 08/06/24 Tuesday [x] streak/patch/innoculate 2007 and 2008 registry parts (13:00 - 14:00) [x] re-transform one sample from yesterday and PocI (13:00) - No growth...) (top)
15:33, 16 October 2008 (diff | hist) N Waterloo/23 June 2008 ‎ (New page: 08/06/23 Monday Morning [x] Mini prep cultures from Sunday (2007 and 2008 registry) 10:00 - 12:30 - 2007 done, but need to redo 2008 miniprep on Wednesda...) (top)
15:25, 16 October 2008 (diff | hist) N Waterloo/22 June 2008 ‎ (New page: 08/06/22 Sunday [x] Inoculated 07 registry parts we need and subcultured cultures from Thursday's transformations (they're all mixed together in the 37, but the transformation...) (top)
15:23, 16 October 2008 (diff | hist) N Waterloo/21 June 2008 ‎ (New page: 08/06/21 Saturday [x] Moved patch/streak plates to 4 degree (they're perched precariously in the igem box) innoculate using cultures from streaked plate (?) and also in...) (top)
15:17, 16 October 2008 (diff | hist) N Waterloo/20 June 2008 ‎ (New page: 08/06/20 Friday To do: [x] Make competent cells [x] Mini prep PluxR-gfp" [x] Diagnostic digest of mini preps (and run on gel) - 1 + 1.5 hr - I think all tr...) (top)
15:10, 16 October 2008 (diff | hist) N Waterloo/19 June 2008 ‎ (New page: 08/06/19 Thursday To do: [x] Elution of registry parts ( including RecA promoter) - ~4 hrs [x] Transformation (Plac gfp'' (?) and RecA promoter) - overlap...) (top)

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