Team:University of Ottawa/7 July 2008

From 2008.igem.org

Revision as of 20:56, 7 July 2008 by Morto077 (Talk | contribs)

Contents

Today in the lab

Dan and Chris

Miniprep
  • Performed a miniprep on all the innocluated strains
  • Digestion
  • Digested miniprep constructs with HindIII and Atcre with EcoRI
  • Gel
  • Matt

    Gel
  • A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
  • The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
  • Performing an inoculation again with new colonies chosen from plates.