The leading idea behind the UniBO Team was to put in touch students of the same University with different backgrounds to favor competence and skill sharing. Students from different Faculties (Biotechnology, Pharmacy and Engineering) answered to the iGEM call and formed the TEAM. They were moved by a common desire for new experiences and they decided to participate in this exciting competition to go deep in Synthetic Biology. The Team worked hard during the summer to become fully operative, overcoming differences both in training and personality.

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The aim of this work area is to determine the characteristics of the promoters that form the circuit. In this section are also collected a brief description of our model, all the equations that describe analytically our circuit, the most meaningful graphics and the results of simulations. Enjoy yourself!

Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this page.

To obtain regulated promoters we synthesized four libraries of operator sequences, respectively for LacI, TetR, cI and LexA repressor proteins. Here is explained how we designed the operator libraries and isolated single operators to clone them in the standard format. Moreover, we built and tested: a LacI regulated promoter to tune promoter activation and a lexA based promoter to study the SOS activation (UV and hydrogen peroxide).So wet!