Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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Results and Discussion
Reaction temparature:37°C,09/12
Sender culture:1000μL,Receiver culture:1000μL
Sender culture:100μL,Receiver culture:1000μL
- Fig. 1,Fig. 2
Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010. Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.
Reaction temparature:37°C
Sender culture:500μLm,Receiver culture:500μL
- Fig. 3
Response time and final fluorescence intensity showed no significant difference. We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.
- Fig. 4
No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Reaction temparature:30°C
Sender culture:500μL,Receiver culture:500μL
- Fig. 6
No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Sender culture:100μL,Receiver culture:1000μL
No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
Sender culture:10μL,Receiver culture:1000μL
The final fluorescence intensity of the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.
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