Brown: Team Resistance/13 June 2008

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13 June 2008

Attempted experimentation with lysis reagent Ran into problems with apparatus: how to put in shaker and not lose solution from plates?

Need apparatus that will withstand shaker

Meeting with Daniel Ludwig at 3pm—will see what suggestions he has Daniel Ludwig suggested trying different concentrations of cells and find that THRESHOLD!

  • 4 electrode system: might want to apply an AC circuit: high frequency is better
  • DAq card only measures voltages but can use labview programming to output a resistance reading
  • Bread-board from barus and holly (Professor J.D. Daniels)

To concentrate bacteria: Count the cells in a certain volume (with microscope) and extrapolate cell density--- concentrate accordingly

  • Method very tedious

For help with Labview etc: Professor J.D. Daniels (engineering), Daniel Ludwig

Suggestion of 4 electrode system: is it completely necessary? Would the voltage drop resulting from copper wires/electrodes be significant? If we use a DC or even AC because it’s such a small wire.

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