Team:Harvard/Plasmids

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Vectors

Parts

  • [http://spreadsheets.google.com/ccc?key=pwvxAM8xwIq37aJ-qKhmUag&hl=en Google Spreadsheet]
Name Registry Name Description Origin Size Marker Transformed? Miniprepped? Culture in Glycerol Stock? Sequenced?
P1 [http://partsregistry.org/Part:BBa_I20259 J23113/I20259]Low promoter GFP testerp15A pMR101-derived919Kanin S1 and TOP10Yes for both strains Yes for both strains.
P2 [http://partsregistry.org/Part:BBa_I20269 J23150/I20269]Medium promoter GFP testerp15A pMR101-derived919Kanin TOP10, not S1
P3 [http://partsregistry.org/Part:BBa_I20270 J23151/I20270]High promoter GFP testerp15A pMR101-derived919Kanin TOP10 and S1Yes for both strains.Yes for both strains.
P4 [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]Low-Medium copy vectorp15a4008CmYesin S1 and TOP10
P5 [http://partsregistry.org/Part:pSB3K3 pSB3K3]Low-Medium copy vector (w/ death gene)p15a2750Kan
P6 [http://partsregistry.org/Part:BBa-E1010 BBa-E1010]RFP only (w/ death gene)pMB1681Kan
P7 [http://partsregistry.org/Part:pSB1A2 pSB1A2]GFP onlypMB12079Amp
P8 [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]RFP with LacI promoterpMB11069Amp
P9 [http://partsregistry.org/Part:BBa_J04430 BBa_J04430]GFP with LacI promoterpMB11083Amp
P10 [http://partsregistry.org/Part:BBa_I715038 BBa_I715038]T7 Polymerase with LacI promoterpMB12878Amp
P11 [http://partsregistry.org/Part:BBa_M30109 BBa_M30109]Light responsive system, dual regulationpUC19-derived pMB14333Amp/Cm
P12 [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pETDuet-1]Low copy plasmidpBR322-derived ColE15420Amp
P13 [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCDFDuet-1]Low copy plasmidCloDF133781SmYesin S1
P14 [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCOLADuet-1]Low copy plasmidColA3719Kan
P15 [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]GFP with Tet promoterColE1937Amp
P16 [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]Tet repressible promoterpMB154Amp
P17 [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400]Inverter (TetR w/o promoter and Tet promoter)OriS, P1 lytic, F1902Kan
P18 [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]lambda promoter (cI regulated)pMB149Amp
P19 [http://partsregistry.org/Part:BBa_I763007 BBa_I763007]RFP with lambda promoterpMB1918Amp
P20 [http://partsregistry.org/Part:BBa_J04431 BBa_J04431]GFP (LVA tagged) with Lac promoterpMB11122Amp, Kan
P21 A1 (pACYC)pACYC (P4) w/ biobricks?????????Yesin S1 and TOP10
P22 [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]ompR controlled promoter for ompC (long)pMB1108Amp
P23 [http://partsregistry.org/Part:BBa_R0083 BBa_R0083]ompR controlled promoter for ompC (short)pMB178Amp
P24 [http://partsregistry.org/Part:BBa_R0084 BBa_R0084]ompR controlled promoter for ompFpMB1108Amp
P25 [http://partsregistry.org/Part:BBa_J58102 BBa_J58102]Promoter activated by OmpR-P with the reporter GFPpUC19-derived pMB1991Amp
P26 [http://partsregistry.org/Part:BBa_J58103 BBa_J58103]Mutated promoter activated by OmpR-P with the reporter GFPpUC19-derived pMB1961Amp
P27 pZS4int2repressor plasmidpSC101SmYes
P28 pZE21GFPColE1KanYes
P29 pZS*2RVenus (mutant YFP)pSC101*KanYes
P30 pZA32YFPp15ACmYes
P31 pZS*1RVenuspSC101*AmpYes
P32 pZA32Venusp15ACmYes
P33 pZA31Venusp15ACmYes
P34 pZE12GFPColE1AmpYes
P35 pLDR8Yes
P36 [http://partsregistry.org/Part:BBa_I763004 BBa_I763004]GFP + IPTG + LVAKan/Amp
P37 [http://partsregistry.org/wiki/index.php/Part:BBa_I722007 BBa_I722007]Constructive expression LacI w/ pTetR promoterAmp
P38 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114]High constitutive promoterAmp
P39 [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa_J23113]Low constitutive promoterAmp
P40 [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032]Ribosome Binding SiteAmp
P41 [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010]Transcriptional TerminatorAmp
P42 [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012]LacI coding regionAmp
P43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040]TetR coding regionAmp
P44 [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051]cI lambdaAmp
P45 [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]GFP only w/ RBS & terminatorAmp
P46 [http://partsregistry.org/Part:BBa_I51020 BBa_I51020]Base VectorAmp
P47 [http://partsregistry.org/Part:BBa_P1003 BBa_P1003]Kan Resistance CassetteKan
P48 [http://partsregistry.org/Part:BBa_P1004 BBa_P1004]Cm Resistance CassetteCm
P49 [http://partsregistry.org/Part:BBa_P1002 BBa_P1002]Amp Resistance CassetteAmp
P50 [http://partsregistry.org/Part:BBa_P1005 BBa_P1005]Tet Resistance CassetteTet
P51 [http://partsregistry.org/Part:BBa_Q04121 BBa_Q04121]LacI QPI w/ RBS & promoterKan
P52 [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]cI Lambda QPIKan
P53 [http://partsregistry.org/Part:BBa_P0440 BBa_P0440]PoPS -> TetRAmp
P54 [http://partsregistry.org/Part:BBa_P0412 BBa_P0412]PoPS -> LacIAmp
P55 [http://partsregistry.org/Part:BBa_P0455 BBa_P0455]RBS.cI.LVA.TermAmp/Cm