Search results

From 2008.igem.org

Create the page "Standard Media" on this wiki!

Brown: Team Resistance/14 July 2008
*Lyse cells in minimal media instead of LB because the ion content of minimal media is controlled and known *use standard copper wire in the breadboard for the circuit and only use gold/platinum fo

5 KB (771 words) - 18:58, 29 October 2008
Teachtheteachers
10:00 am -- Jason Kelly -- Standard Measurement Kit for BioBrick Promoters (PoPS-out devices) ...xplains how to measure the PoPs of promoters and compare it to a reference standard.

5 KB (778 words) - 20:34, 8 May 2008
Team:ETH Zurich/Project/Motivation
...ard E. coli, the cells with reduced genome grew slightly denser in certain media, tolerated a greater variety of plasmids and had higher electroporation eff

6 KB (907 words) - 09:57, 30 October 2008
Team:Caltech/Protocols/Measuring H2O2
...(100uM, 33.3uM, 11.1 uM, 3.7 uM and 0.0 uM H2O2) made in the corresponding media. [[Image:H2O2 standards example.png|thumb|center|400px|An example of a typical standard curve generated by the assay. Error bars (1SD, n=3) are too small to be see

1 KB (162 words) - 00:24, 30 October 2008
SOB
[[Category:Material]] [[Category:Standard Media]]

1 KB (171 words) - 02:13, 31 May 2008
Team:Illinois/Antibody RTK Fusion
...pplies needed will include the cholera B subunit, yeast culture media, and standard molecular biology reagents/kits.

7 KB (1,026 words) - 04:36, 30 October 2008
Team:Freiburg/Project
...he construction of fusion proteins due to extended Biobrick 3.0 (Freiburg) standard. Cloning was done in E. coli. However, we test our receptors in huma ...Origami-yield and measurement of the molecule´s stability in cell culture media the later ones. 

12 KB (1,733 words) - 08:24, 30 October 2008
Team:Illinois/Antibody GPCR Fusion Notebook
** Brought to standard concentration, 30uM *Add SOC Media (200uL)

13 KB (1,969 words) - 02:55, 30 October 2008
Team:TUDelft/Ethics conclusions
'''Full report: Click this [[Media:TUDelft_iGEM_Ethics_StevenF.pdf|link]]''' ... involved and hence the good products will not be added to the registry of standard biological parts.

20 KB (3,152 words) - 19:44, 29 October 2008
Team:Chiba/Sender experiments
#Inoculated them independently in liquid media. Incubated at 37°C 12h ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth

6 KB (641 words) - 12:14, 29 October 2008
Team:Brown/Notebook/Timeline
...d, our apparatus became more complex. We quickly made the transition from standard voltmeter to a Data Acquisition Card coupled with LabView Computer Software *We also experimented with LB Lennox, Luria, M9 Minimal Media, Distilled/Sterile Water, as well as Milli-Q water. Cells were pelleted an

6 KB (917 words) - 17:35, 29 October 2008
Team:Heidelberg/Notebook/Killing I/Notebook/week12
* the insert was ligated in the lambda backbone over night using standard ligation methods * colonies were picked and transferred in liquid media containing chloramphenicol

36 KB (4,978 words) - 14:47, 29 October 2008
Team:Heidelberg/Human Practice/Phips the Phage/Technical Backround
...transformed bacteria to survive, if antibiotics are present in the culture media. All untransformed cells die due to the added antibiotics, because they do ...itcal introduction, the pupils carry out a plasmid minipreparation using a standard kit.

51 KB (8,118 words) - 16:56, 29 October 2008
Team:Imperial College/B.subtilis
... subtilis chassis page</a></html> on the '''Registry of Biological Standard Parts'''. We characterised the growth rate of ''B. subtilis'' in LB media at 37oC. This curve provides useful information when inducing pr

3 KB (492 words) - 03:41, 30 October 2008
Team:Slovenia/Notebook/Methods
...r the overexpression of flagellar proteins. Cultures were grown in LB media (Luria-Bertani) supplemented with ampicillin at 37°C with shaking ...dividually numbered by ear-notching, regularly examined, weighed and fed a standard rodent chow (Altromin 1324, Lage, Germany). Food was available ad libitu

48 KB (6,976 words) - 04:42, 30 October 2008
Team:Davidson-Missouri Western/Viz-A-Brick: A New Way to Visualize the Registry
As the Registry of Standard Biological Parts expands, it becomes increasingly important that users be a [[Media:Example.ogg]]

3 KB (484 words) - 18:13, 29 October 2008
Team:Chiba/Project/Experiments:Crosstalk
...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth #Inoculated them independently in liquid media. Incubated at 37°C 12h

7 KB (579 words) - 12:43, 29 October 2008
Team:Cambridge/Voltage/Lab Work
...increase volume in preparation for experiments tomorrow on K+ tolerance in media. * Designed standard infusion primers for biobrick assembly. Abandoned due to unfeasibly large s

21 KB (3,118 words) - 23:31, 29 October 2008
Team:Chiba/Project/Experiments:Sender Crosstalk
#Inoculated them independently in liquid media. Incubated at 37°C for 12hours. ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI, RhlI, and LasI means fluorescence induced by AHLs

7 KB (1,039 words) - 11:00, 30 October 2008
Team:Groningen/references.html
...ttp://openwetware.org/wiki/Griffitts:Common_media">Openwetware.org: Common Media</a></li> ...tp://partsregistry.org/Assembly:Standard_assembly">Partsregistry.org Help: Standard Assembly</a></li>

7 KB (924 words) - 00:22, 30 October 2008