User contributions
From 2008.igem.org
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- 18:29, 26 October 2008 (diff | hist) User:Qiqin
- 18:28, 26 October 2008 (diff | hist) N User:Qiqin (New page: ==Contact Info== *Qi, Qin *Imperial College of Science, Technology and Medicine I'm a member of IGEM:IMPERIAL/2008 at Imperial College. ==Education== <!--Include info about your ed...)
- 18:25, 26 October 2008 (diff | hist) Team:Imperial College/Constitutive
- 18:22, 26 October 2008 (diff | hist) Team:Imperial College/Light Inducible
- 18:21, 26 October 2008 (diff | hist) Team:Imperial College/Inducible
- 18:21, 26 October 2008 (diff | hist) Team:Imperial College/Fluorescence
- 18:21, 26 October 2008 (diff | hist) Team:Imperial College/Constitutive
- 18:20, 26 October 2008 (diff | hist) Team:Imperial College/Constitutive
- 18:20, 26 October 2008 (diff | hist) Team:Imperial College/Growth
- 18:18, 26 October 2008 (diff | hist) Team:Imperial College/Protocols
- 18:18, 26 October 2008 (diff | hist) Team:Imperial College/XL1 Blue
- 18:17, 26 October 2008 (diff | hist) Team:Imperial College/Transformation 2
- 15:02, 26 October 2008 (diff | hist) Team:Imperial College/Fluorescence
- 15:01, 26 October 2008 (diff | hist) Team:Imperial College/Constitutive
- 15:00, 26 October 2008 (diff | hist) N File:Spectra YtvA.PNG (top)
- 14:59, 26 October 2008 (diff | hist) N Team:Imperial College/Light Inducible (New page: {{Imperial/StartPage2}} __NOTOC__ =Characterization of Light Inducible Expression= ===Status=== Feedback wanted ===Aims=== The aim of this protocol is to characterise the light induced exp...)
- 14:59, 26 October 2008 (diff | hist) N Team:Imperial College/Inducible (New page: {{Imperial/StartPage2}} __NOTOC__ =Initial Characterization of pHyperspank Inducible Promoter= ===Status=== Feedback wanted ===Aims=== *The aim of this protocol is to help us define the d...)
- 14:58, 26 October 2008 (diff | hist) N Team:Imperial College/Constitutive (New page: {{Imperial/StartPage2}} __NOTOC__ =Characterization of Constitutive Expression= ===Aims=== *To characterise the constitutive promoter and RBS combinations. This protocol is concerned with...)
- 14:57, 26 October 2008 (diff | hist) N File:GFP plate reader.PNG (top)
- 14:57, 26 October 2008 (diff | hist) N Team:Imperial College/Fluorescence (New page: {{Imperial/StartPage2}} __NOTOC__ =GFPmut3b Fluorescence vs molecules of GFPmut3b= ===Aims=== *The aim of this protocol is to produce a calibration curve of fluorescence vs molecules of G...)
- 14:56, 26 October 2008 (diff | hist) N Team:Imperial College/Growth (New page: {{Imperial/StartPage2}}__NOTOC__ =Cell Count v Optical Density Curve Calibration= ===Aim=== To produce a calibration curve to aid in the normalising of fluorescence values to allow prope...)
- 14:54, 26 October 2008 (diff | hist) N File:Calibration Curve5.JPG (top)
- 14:54, 26 October 2008 (diff | hist) N File:Calibration Curve4.JPG (top)
- 14:54, 26 October 2008 (diff | hist) N File:Calibration Curve3.JPG (top)
- 14:54, 26 October 2008 (diff | hist) N File:Calibration Curve2.JPG (top)
- 14:53, 26 October 2008 (diff | hist) N File:Absorbance calibration curve.jpg (top)
- 14:51, 26 October 2008 (diff | hist) N Team:Imperial College/Calibration Curves (New page: {{Imperial/StartPage2}} __NOTOC__ ==Calibration Curves== *The two measurements that will be made for the various devices are fluorescence emission and absorbance at 600nm. * The aim of the...)
- 14:50, 26 October 2008 (diff | hist) N Team:Imperial College/PCR (New page: {{Imperial/StartPage2}} __NOTOC__ ==PCR== This protocol is desgined for use with the stratagene ''PfuUltra'' II Fusion DNA polymerase and is based in part on the ''PfuUltra'' II Fusion D...)
- 14:49, 26 October 2008 (diff | hist) Team:Imperial College/Transformation 2
- 14:49, 26 October 2008 (diff | hist) Team:Imperial College/XL1 Blue
- 14:48, 26 October 2008 (diff | hist) N Team:Imperial College/XL1 Blue (New page: {{Imperial/StartPage2}} ==Preparation of XL1-Blue Electrocompetent cells== ===Aims=== Preparation of ''E. coli'' cells for the cloning of Biobricks and construct construction ===Equipme...)
- 14:47, 26 October 2008 (diff | hist) Team:Imperial College/Transformation 2
- 14:46, 26 October 2008 (diff | hist) Team:Imperial College/Transformation
- 14:44, 26 October 2008 (diff | hist) Team:Imperial College/Transformation
- 14:44, 26 October 2008 (diff | hist) Team:Imperial College/Transformation
- 14:43, 26 October 2008 (diff | hist) N Team:Imperial College/Transformation 2 (New page: {{Imperial/StartPage2}} =Transformation Protocol 2= ===Equipment=== *Centrifuge, *Water bath ===Reagents and Materials=== -'''''SOC broth, per litre:''''' - 10ml <br> *2% tryptone (20g) *0...)
- 14:42, 26 October 2008 (diff | hist) N Team:Imperial College/Transformation (New page: {{Imperial/StartPage2}} __NOTOC__ =Transformation Protocol 1= ==Equipment== ==Reagents== '''''SpII:''''' *200ml T base, *50% (w/v) glucose 2ml, *1.2% (w/v) MgSO<sub>4</sub>.7H<sub>2</sub...)
- 14:41, 26 October 2008 (diff | hist) Team:Imperial College/Protocols
- 14:39, 26 October 2008 (diff | hist) Imperial College/Transformation 2 (Removing all content from page) (top)
- 14:39, 26 October 2008 (diff | hist) Imperial College/Transformation (Removing all content from page) (top)
- 14:38, 26 October 2008 (diff | hist) Team:Imperial College/Protocols
- 14:37, 26 October 2008 (diff | hist) N Imperial College/Transformation 2 (New page: {{Imperial/StartPage2}} =Transformation Protocol 2= ===Equipment=== *Centrifuge, *Water bath ===Reagents and Materials=== -'''''SOC broth, per litre:''''' - 10ml <br> *2% tryptone (20g) *0...)
- 14:36, 26 October 2008 (diff | hist) Team:Imperial College/Protocols
- 14:36, 26 October 2008 (diff | hist) N Imperial College/Transformation (New page: {{Imperial/StartPage2}} __NOTOC__ =Transformation Protocol 1= ==Equipment== ==Reagents== '''''SpII:''''' *200ml T base, *50% (w/v) glucose 2ml, *1.2% (w/v) MgSO<sub>4</sub>.7H<sub>2</sub...)
- 14:36, 26 October 2008 (diff | hist) Team:Imperial College/Protocols
- 14:35, 26 October 2008 (diff | hist) /Transformation (Removing all content from page) (top)
- 14:33, 26 October 2008 (diff | hist) N /Transformation (New page: {{Imperial/StartPage2}} __NOTOC__ =Transformation Protocol 1= ==Equipment== ==Reagents== '''''SpII:''''' *200ml T base, *50% (w/v) glucose 2ml, *1.2% (w/v) MgSO<sub>4</sub>.7H<sub>2</sub...)
- 11:35, 26 October 2008 (diff | hist) Team:Imperial College/Summary
- 11:34, 26 October 2008 (diff | hist) Team:Imperial College/Summary
- 11:33, 26 October 2008 (diff | hist) Team:Imperial College
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