Team:University of Ottawa/27 June 2008

From 2008.igem.org

Revision as of 17:26, 1 July 2008 by Djedrysiak (Talk | contribs)

Dan

Ligation
  • Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
  • Components New Name
    0B + 0A 1
    0B + 1A 2
    1B + 0A 3
    1B + 1A 4
  • Gel indicated that no ligation occured. There are several reasons for this:
    1. We only have a 2 bp overhang
    2.We are not using enough enzyme
    3. We did not use enough buffer
  • Enough buffer was added and enzyme was doubled, left to react over the weekend