User:University of Washington/8 July 2008

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BioBrick Promoter Measurements

- VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water.

- 8 uL of diluted VF2 primer (1pmol/uL) was added to Eppendorf tubes.

- 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriately labeling the Eppendorf tubes.

- Plasmid template and VF2 primer solutions were supplied to the UW DNA Sequencing Facility for reaction and analysis.


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