Team:Rice University/Notebook/18 July 2008

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Friday, July 18th

  • David Ouyang
    • Miniprep R0040 (ptet)
    • Digest Amber Suppressor and ptet
    • Ligate together
    • Do ARFP mutant quikchange and DPN1
    • Transform ARFP and Amber Suppressor supposed
  • Taylor Stevenson
  • Repeat PCR from yesterday using these primers (NOTE: to view primers download this link and change extension to .doc (word file)[1]).
    1. tried raising annealing temperature to 58*C

20080718.jpg (100bp standard, BleoC PCR product, Stf PCR product,Ligation of yesterdays digested and CIPed PCR primers, 1kb standard)



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