Team:Paris/August 2

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Results of the transformation

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100 rbs TetR - ECFP


D110 (BV) - D130 (BI)

To do again
L101 rbs TetR - GFP tripart


D110 (BV) - D131 (BI)

To do again
L102 Strong rbs - YFP


D129 (BV) - D118 (BI)

To do again
L103 Strong rbs - GFP


D129 (BV) - D122 (BI)

To do again
L104 Strong rbs - lasR activator


D129 (BV) - D114 (BI)

To do again
L105

Strong promoter - ECFP
D123 (BV) - D130 (BI)

OK
L106

Strong promoter - gfp Tripart
D123 (BV) - D131 (BI)

OK
L107

Strongest promoter - ECFP
D103 (BV) - D131 (BI)

To do again
L108 n°2 (the right one)

Strong promoter - gfp Tripart
D103 (BV) - D130 (BI)

OK
L110

Medium promoter - gfp Tripart
D124 (BV) - D131 (BI)

OK
L111

Weak promoter - ECFP
D104 (BV) - D130 (BI)

To do again
L112

Weak promoter - gfp
D104 (BV) - D131 (BI)

To do again
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

To do again
L115

pLas - ECFP
D105 (BV) - D130 (BI)

To do again
L116

pLas - gfp Tripart
D105 (BV) - D131 (BI)

To do again
L117

yfp - Double Terminator
D125 (FV) - D117 (FI)

To do again
L118

rfp - Double Terminator
D125 (FV) - D121 (FI)

To do again
L119

lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI)

To do again
L120

tetR repressible - ECFP
D106 (BV) - D130 (BI)

To do again
L121

Strong promoter - gfp tripart
D106 (BV) - D130 (BI)

OK
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

To do again
L124

Strongest RBS - rfp
D102 (BV) - D122 (BI)

To do again
L125

Strongest RBS - yfp
D102 (BV) - D118 (BI)

To do again L126

Strongest RBS (1)- LacR activator (+LVA)
D102 (BV) - D118 (BI)

To do again


Results of the transformation (bis-the good one to leave in august 2)

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

608 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

> 4000 GFP positive OK
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

9 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

9 To do again
L120

tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)

74 To do again
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

4 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

> 4000 To do again
L126

Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)

944 To do again