Team:Paris/August 16

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Analysis of the transformation we did yesterday

L143, L144, T1 and T2 showed no colonies. The positive control with pUC19 worked well.
We suppose that the ligation did not work. We will do it again today.

Ligation

Ligation

Cleaning of the DNA after the digestion

Measure of DNA concentration of the digestion products

List of ligations

Protocol

Transformation

Transformation protocol

Name Description Antibio
Ligations
L150 D105(BV) - D146(BI)
pLas - strongest rbs-TetR-GFP tripart
Amp
L151 D147(FI) - D125 (FV)
strongest rbs-LasR activator with LVA - Double terminator
Kan
Controls
C1 D105 Amp
C2 D125 Kan
Positive Control pUC19 Amp