Illinois/31 July 2008

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Receptor Tyrosie Kinase Group

PCR to asemble fragments from yesterday.

tube 1 2 3 4
template 0.5ul of both heavy and light chains
primers 1ul of appropriate forward and reverse primer
MgCl2 0ul 0ul 3ul 3ul
master mix 20ul
H20 to 50ul total volume

PCR program: 1. 94 degrees 5 min 2. 94 degrees 1 min 3. 50 degrees 1 min 4. 72 degrees 1 min 5. GOTO 2. 29 cycles 6. HOLD at 4 degrees

Products run on a 1.5% agarose gel, no products of 700-800bp.

7 31 08 assembly.jpg