Preparation of constructs with OmpA protein fusions
1. Colony PCR on colonies from plates with transformations OmpA_alpha.
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin. Cloning of protein Z DNA to OmpA constructs
1. 2 colonies was inoculated to liquid LB broth with kanamycin Preparation of construct omega-A
1. PCR A in 50 µl
template DNA - pKS-A4 1 µl
primer
AP+NotI_N - 2 µl
primer
AL+link10+homo2_N - 2 µl
Pfu polymerase buffer + Mg2+ (from Fermentas) - 5 µl
dNTPs - 1 µl
Pfu turbo - 0.5 µl
H2o - 38.5 µl