July

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__july

Assess Pool

We mixed all oligos together accept the modified, the left and the right borders and the remainders.

1) Oligos modified with flourophor:

1. board:

r-3t8f
r-5t10e
r-5t12e

2) Oligos modified with NIP:

1. board:

r-7t6f
r-7t8f
r-7t10f
r-5t8f
r-3t10e
r-7t6e

3. board:

r7t22e

3) Left border: 1.board, 1.line
4) Right border: 3.board, 2.line
5) Remainders: 3.board, 3.line

Assess cell-culture (DYT-Medium; ER 2738-cells)

50ml DYT-Medium
+ 50 TET (25ng/ml -> 1:1000)
+ pick ER 2738-cells (from -80 degree freezer)

-> shake at 37 degree over night