"
Brown: Team Resistance/9 July 2008
From 2008.igem.org
- OPTICAL DENSITY TEST:
- We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
- Initial OD 50mL culture: .038
.2%(1) .2%(2) .4%(1) .4%(2) .2% + Chloramphenicol(1) .2% + Chloramphenicol(2) Control
- 3:45 PM .084 .085 .079 .079 .062 .053 .073
- 4:45 PM .065 .069 .040 .048 .044 .047 .076
- 5:55 PM .018 .018 .023 .021 .034 .050 .075
- 6:50 PM .014 .015 .013 .013 .050 .044 .071
- The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated.
- The addition of Chloramphenicol did not alter the rate of lysis.
