Team:Tokyo Tech/Genetic toggle switch to implement rewritable function

From 2008.igem.org

Revision as of 20:52, 22 October 2008 by Ryouichi (Talk | contribs)
Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry The Team Notebook


Genetic toggle switch to implement rewritable function

We think two type models - genetic circuit model and mathematical model.

Genetic circuit model

Tech 4toggle1.JPG

fig5-1:genetic circuit model


1. Writable function

・ Plac is induced under 30MPa pressure(PL is weakly induced)

・ Express CI and GFP

・ CI represses PL and causes low LacI concentration

・ Express increasingly CI and GFP ⇒ Bright!!


2. Erasable function

・PL is induced by heat

・Express LacI and LacI represses Plac

・Therefore, GFP expression decreases

Mathematical model