Team:Bologna/Notebook

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HOME PROJECT TEAM SUBMITTED PARTS MODELING WETLAB LAB-BOOK BIOSAFETY AND PROTOCOLS




Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

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Contents

Protocols

For more information, see Biosafety page
Protocol Biosafety level
Plates preparation
1
Amplification
1
Transformation
1
Inoculation
1
Miniprep
1
Digestion reaction
1
Gel preparation
2
Electrophoretic run
2
Gel extraction
2
Ligation reaction
1
Chemiocompetent cells
1
Mediums and buffers
1
Antibiotics stocks preparation
1
IPTG stocks preparation
1
Fluorescence test
1
M9 supplemented media
1



Week 1: from 07/21/08 to 07/27/08

General Preparations

  1. Preparation of chemiocompetent cells from E. Coli DH5α, Top10 and DB 3.1
  2. Preparation of antibiotic stocks for Ampicillin and Kanamicin
  3. Preparation of LB medium and LB plates for cloning.

Week 2: from 07/28/08 to 08/03/08

  1. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_R0082 /R0082, [http://partsregistry.org/Part:BBa_R0083, R0083], [http://partsregistry.org/wiki/index.php/Part:BBa_M30109 /M30109] in TOP10 strain to build and characterize the Light response system to be our spatial selective trigger.
  1. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_E0240 /E0240, [http://partsregistry.org/Part:BBa_P1010 /pSB3K3_P1010] and the Practice Promoter Set ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23103] /J23150, J23151, J23102) to test and set up the new [http://partsregistry.org/Measurement /Biobrick Standard Measurement Protocol]




  1. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240

Week 3: from 08/04/08 to 08/10/08

Week 4: from 08/11/08 to 08/17/08

Week 5: from 08/18/08 to 08/24/08

Week 6: from 08/25/08 to 08/31/08

Week 7: from 09/01/08 to 09/07/08

Week 8: from 09/08/08 to 09/14/08

Week 9: from 09/15/08 to 09/21/08

Week 10: from 09/22/08 to 09/28/08

Week 11: from 09/29/08 to 10/05/08

Week 12: from 10/06/08 to 10/12/08

Week 13: from 10/13/08 to 10/19/08

Week 14: from 10/20/08 to 10/26/08

Week 15: from 10/27/08 to 10/29/08



















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