Team:Bologna/Parts

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HOME PROJECT TEAM SUBMITTED PARTS MODELING WETLAB LAB-BOOK BIOSAFETY AND PROTOCOLS


Composites

The table below shows all the composites that we have obtained to build up our final device. For each part you can find:

-in the second column the link to the Registry;

-in the third column the components for each part;

-in the fourth column the plasmid the part has been cloned in;

-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;

-in the sixth column cell vitality after transformation;

-in the seventh column fluo data for each part.


Igem Code Fragment Plasmid Length (bp) Vitality Fluo
1
[http://partsregistry.org/Part:BBa_K079000 K079000] LacI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1290
Yes
-
2
[http://partsregistry.org/Part:BBa_K079001 K079001] LacI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 2075
Yes
3
[http://partsregistry.org/Part:BBa_K079002 K079002] RBS-LacI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1308
Yes
-
4
[http://partsregistry.org/Part:BBa_K079003 K079003] RBS-LacI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 2093
Yes
5
[http://partsregistry.org/Part:BBa_K079004 K079004] TetR-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1607
Yes
7
[http://partsregistry.org/Part:BBa_K079006 K079006] RBS-TetR-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1625
Yes
8
[http://partsregistry.org/Part:BBa_K079007 K079007] RBS-TetR-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 840
Yes
-
9
[http://partsregistry.org/Part:BBa_K079008 K079008] CI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 912
Yes
-
10
[http://partsregistry.org/Part:BBa_K079009 K079009] CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3(HC)] 1697
Yes
11
[http://partsregistry.org/Part:BBa_K079010 K079010] RBS-CI-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 930
Yes
-
12
[http://partsregistry.org/Part:BBa_K079011 K079011] RBS-CI-RBS-GFP-T [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2(HC)] 1715
Yes
13
O1Lac
-
14
O2Lac
-
15
O3Lac
-
16
O1Tet
-
17
O2Tet
-
18
O3Tet
-
19
O1Lambda
-
20
O2Lambda
-
21
O3Lambda
-
22
O1LexA
-
23
O2LexA
-
24
O3LexA
-
25
O2Lac-O1Lac
-
26
O2Tet-O1Tet
-
27
O2Lambda-O1Lambda
-
28
O2LexA-O1LexA
-
29
O3Lac-O2Lac-O1Lac
-
30
O3Tet-O2Tet-O1Tet
-
31
O3Lambda-O2Lambda-O1Lambda
-
32
O3LexA-O2LexA-O1LexA
-
33
O1Lac-RBS-LacI-T
-
34
O2Lac-RBS-LacI-T
-
35
O3Lac-RBS-LacI-T
-
36
O1Lac-RBS-TetR-RBS-GFP-T
-
37
O2Lac-RBS-TetR-RBS-GFP-T
-
38
O3Lac-RBS-TetR-RBS-GFP-T
-

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