Team:Warsaw/Calendar-Main/23 July 2008
From 2008.igem.org
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPawełLigations transformed into TOP10 and plated on LB + ampicillin. Cloning of protein A DNA to OmpA constructsMichał K.
AntoniSetup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria. Fig. 1. Control SacI/BamHI digests of pACYC177+OmpA_A_alpha1-4. digested plasmids isolated from transformants obtained previous day 5. Marker
|