Team:Warsaw/Calendar-Main/5 August 2008
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Cloning of truncated fragment of protein A (ΔA)Emilia
Checking if OmpA_omega_ΔA_alpha gives ampicillin resistancePiotrInoculation OmpA_omega_ΔA_alpha from various IPTG concentrations (in mmol/mL) (0, 0.1, 0.25, 0.5, 0.75, 1) into same IPTG concentrations, but with various ampicillin concentrations (in mmol/mL)(25, 50, 75, 100) in ratio: 1:50. Measurement of bacterial culture growth (OD) in the evening:
Inoculation of OmpA_omega_ΔA_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75 mmol/mL (repetition is necessary) Checking OmpA_omega_ΔA_alpha and OmpA_A_alpha expressionPiotr
1 - protein marker, 2 and 3 - E coli without plasmid, 4 - Omp_omega_A_alpha + 0,5 mM IPTG, 5 - E coli without plasmid, 6 - Omp_omega_A_alpha + 0,5 mM IPTG, 7 - Omp_omega_A_alpha without inducer. Preparing pACYC177+OmpA_omega_ΔA constructMichał K.
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