Team:Hawaii/Notebook/2008-06-19

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Things we did today

Wetlab work

Alkaline Lysis Miniprep

Krystle & Margaret
  • Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101).

Cryo-Stocks

Krystle
  • Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.

Drylab Work

Primer Discussion

Krystle, Norman, & Margaret
  • Discussed the format of primers
  • worked on revising primers


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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