Team:Michigan/Project
From 2008.igem.org
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Project DescriptionCircadian ClocksShort background on circadian clocks... why they're important, why they're studied, maybe who studies them... Our Project: The SequestillatorWe can subdivide our clock into three parts: the tetR system, the activator module, and the repressor module. The activator module consists of the tet promoter driving the NifA gene. The NifA protein binds to the nifHp promoter of the repressor module, activating transcription of the NifL gene. Once NifL dimerizes, it can bind to the NifA hexamer, hence preventing NifA from binding to NifHp. This sequestration effect provides the clock's negative feedback loop that is essential for oscillations. The tetR system regulates the levels of nifA. A constitutive promoter drives the TetR gene. At its resting state of our circuit, TetR completely represses the production of NifA. However, addition of anhydrotetracycline prevents the Tet protein from binding to tet promoter, allowing for a "constitutive" production of NifA as long as the levels of anhydrotetracycline are kept constant. This module allows us to tune the levels of NifA by adjusting the amount of anhydrotetracycline we add. |
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Sequestilator Modeling |
Sequestilator FabricationIf you like the way this looks, you could put a summary of what you built here and then we can have a separate page for fabrication, which might be a good idea. |
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