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Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)
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design
Sender
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI(BBa_K084007)]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI(BBa_K084008)]
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
- [http://partsregistry.org/Part:BBa_K084012 BBa_K084012]
- [http://partsregistry.org/Part:BBa_K084014 BBa_K084014]
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623(ptet+rbs+LuxI(LVA))]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Results
Reaction temparature:37°C
- センダーの培養液：500μL、レシーバの培養液：500μL
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Reaction temparature:30°C
- センダーの培養液：500μL、レシーバの培養液：500μL
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Reaction temparature:25°C
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