Team:Michigan/Project/LandingPads

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BioBrick Landing Pads

BioBrick Landing Pad Polylinker

Landing Pads: aid in the insertion of biological constructs onto the chromosome of E. coli

Landing Pad Goals:

  • BioBrick compatibility
  • Easy phenotypic screening
  • Easy sequencing
  • Limit noise

Polylinker Features:

  • BioBrick compatible restriction sites - these allow for standard BioBrick construction in forward and reverse directions
  • Additional building restriction sites - these are for building the landing pad, as well as changing the homologous regions and drug resistance gene (can also be used for building if needed)
  • Bar code sequence - a 20 bp segment that allows for easy sequencing
  • Drug resistance gene - this allows for easy phenotypic screening
  • Homologous regions - these specify where on the chromosome you wish to put your constructs.
Landing Pad Polylinker - gold.png

Landing Pads used for the Sequestilator

Operon



Activator Operon




Repressor Operon

Topology

New 1st operon - gold.PNG


New 2nd operon - gold.PNG

Landing Pad

Leucine Landing Pad

constructed by former Ninfa lab member, Don Eun Chang


Arabinose Landing Pad

iGEM 2007 project by Alyssa Delke & Khalid Miri

stuff about landing pad plasmids


Landing pad plasmid - gold.png





Learn more about Landing Pads [http://synthbio.engin.umich.edu/wiki/index.php/BioBrick_Landing_Pad Here!]




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