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Template:Team:UC Berkeley/Notebook/CDB construction
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PCR ca8373/ca8374 on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
pBca1256-K112400
Biobricking of promoter for grpE protein on MG1655 PCR grpE-F and grpE-R (129 bp, EcoR1/BamH1/Dpn1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112400 ---------------------------- grpE-F Forward Biobricking ccataGAATTCatgAGATCTccgaggtccttgttgcgaaga grpE-R Reverse Biobricking cgttaGGATCCcgcattcatctcgctaacttc
