Alberta NINT/30 May 2008

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lab work (SD): DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol.  This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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lab work (JD): Counted colonies on ligation plates: negative plate:0, Positive plate:2 and because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
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[[Alberta_NINT/29_May_2008 | < Previous entry]] | [[Alberta_NINT/31_May_2008 | Next entry >]]
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lab work (SD):  
 +
 
 +
          DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep  
 +
          protocol.   
 +
          This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
 +
 
 +
 
 +
lab work (JD):  
 +
 
 +
          Counted colonies on ligation plates: negative plate:0, Positive plate:2  
 +
          because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
 +
 
 +
lab work (WM):
 +
          Miniprepped 8 O/Ns
 +
          Digested K102010/(EcoRI + PstI)
 +
          Sequence with VR primer
 +
          [[image: NINT_WMpg24b.JPG|500px]]

Latest revision as of 22:12, 11 October 2008

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lab work (SD):

         DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep 
         protocol.  
         This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.


lab work (JD):

          Counted colonies on ligation plates: negative plate:0, Positive plate:2 
          because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow

lab work (WM):

          Miniprepped 8 O/Ns
          Digested K102010/(EcoRI + PstI)
          Sequence with VR primer
          NINT WMpg24b.JPG