From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
Home >> Wet Lab >> Newcastle Wet Lab Journal
Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
| August
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| M | T | W | T | F | S | S
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| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/1_August_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/2_August_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/3_August_2008&action=edit 3]
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| [http://2008.igem.org/Newcastle_University_Wetlab/4_August_2008 4]
| [http://2008.igem.org/Newcastle_University_Wetlab/5_August_2008 5]
| [http://2008.igem.org/Newcastle_University_Wetlab/6_August_2008 6]
| [http://2008.igem.org/Newcastle_University_Wetlab/7_August_2008 7]
| [http://2008.igem.org/Newcastle_University_Wetlab/8_August_2008 8]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/9_August_2008&action=edit 9]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/10_August_2008&action=edit 10]
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| [http://2008.igem.org/Newcastle_University_Wetlab/11_August_2008 11]
| [http://2008.igem.org/Newcastle_University_Wetlab/12_August_2008 12]
| [http://2008.igem.org/Newcastle_University_Wetlab/13_August_2008 13]
| [http://2008.igem.org/Newcastle_University_Wetlab/14_August_2008 14]
| [http://2008.igem.org/Newcastle_University_Wetlab/15_August_2008 15]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/16_August_2008&action=edit 16]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/17_August_2008&action=edit 17]
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| [http://2008.igem.org/Newcastle_University_Wetlab/18_August_2008 18]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/19_August_2008&action=edit 19]
| [http://2008.igem.org/Newcastle_University_Wetlab/20_August_2008 20]
| [http://2008.igem.org/Newcastle_University_Wetlab/21_August_2008 21]
| [http://2008.igem.org/Newcastle_University_Wetlab/22_August_2008 22]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/23_August_2008&action=edit 23]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/24_August_2008&action=edit 24]
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| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/25_August_2008&action=edit 25]
| [http://2008.igem.org/Newcastle_University_Wetlab/26_August_2008 26]
| [http://2008.igem.org/Newcastle_University_Wetlab/27_August_2008 27]
| [http://2008.igem.org/Newcastle_University_Wetlab/28_August_2008 28]
| [http://2008.igem.org/Newcastle_University_Wetlab/29_August_2008 29]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/30_August_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/31_August_2008&action=edit 31]
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| September
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| M | T | W | T | F | S | S
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| [http://2008.igem.org/Newcastle_University_Wetlab/1_September_2008 1]
| [http://2008.igem.org/Newcastle_University_Wetlab/2_September_2008 2]
| [http://2008.igem.org/Newcastle_University_Wetlab/3_September_2008 3]
| [http://2008.igem.org/Newcastle_University_Wetlab/4_September_2008 4]
| [http://2008.igem.org/Newcastle_University_Wetlab/5_September_2008 5]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/6_September_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/7_September_2008&action=edit 7]
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| [http://2008.igem.org/Newcastle_University_Wetlab/8_September_2008 8]
| [http://2008.igem.org/Newcastle_University_Wetlab/9_September_2008 9]
| [http://2008.igem.org/Newcastle_University_Wetlab/10_September_2008 10]
| [http://2008.igem.org/Newcastle_University_Wetlab/11_September_2008 11]
| [http://2008.igem.org/Newcastle_University_Wetlab/12_September_2008 12]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/13_September_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/14_September_2008&action=edit 14]
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| [http://2008.igem.org/Newcastle_University_Wetlab/15_September_2008 15]
| [http://2008.igem.org/Newcastle_University_Wetlab/16_September_2008 16]
| [http://2008.igem.org/Newcastle_University_Wetlab/17_September_2008 17]
| [http://2008.igem.org/Newcastle_University_Wetlab/18_September_2008 18]
| [http://2008.igem.org/Newcastle_University_Wetlab/19_September_2008 19]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/20_September_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/21_September_2008&action=edit 21]
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| [http://2008.igem.org/Newcastle_University_Wetlab/22_September_2008 22]
| [http://2008.igem.org/Newcastle_University_Wetlab/23_September_2008 23]
| [http://2008.igem.org/Newcastle_University_Wetlab/24_September_2008 24]
| [http://2008.igem.org/Newcastle_University_Wetlab/25_September_2008 25]
| [http://2008.igem.org/Newcastle_University_Wetlab/26_September_2008 26]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/27_September_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/28_September_2008&action=edit 28]
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| [http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/29_September_2008&action=edit 29]
| [http://2008.igem.org/Newcastle_University_Wetlab/30_September_2008 30]
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Friday 15th August
pGFPrrnB, pJWV021 and BBa_I746107 were restricted using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume:
- pGFPrrnB restricted with EcoRI + NheI
- pJWV021 restricted with NheI + BglII
- pGFPrrnB restricted with EcoRI + SpeI
- BBa_I746107 restricted with EcoRI + SpeI
These were incubated at 37˚C for 1 hour and run on a gel. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted.
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