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Jamboree/Project Abstract/Team Abstracts
...to-Builders'', we investigate the possibility of using large numbers of E. coli to perform a task too great for any individual cell. Specifically, this in ''Ecoli.PROM: an Erasable and Programmable Genetic Memory with E. coli''

76 KB (11,186 words) - 15:27, 6 November 2008
Team:NYMU-Taipei/Project/Phosphate
* Enhance and regulate the phosphate absorption of ''E.coli'' to balance phosphate concentration to a normal level in patients with kid === Phosphate Metabolism and Regulation in ''E.coli'' ===

7 KB (967 words) - 05:14, 30 October 2008
Team:University of Sheffield /Project
... cholera pathogens expression of the receptor protein would transform an E.coli into a biosensor. The most straightforward, which does not mean the easiest ...nal conveyer domain of BarA which is a protein present on the surface of E.coli cell and that passes signal downstream to DNA.

22 KB (3,311 words) - 01:07, 29 October 2008
Team:Edinburgh/Plan
== The chassis: ''E. coli'' vs. ''B. subtilis'' == We were initially torn between using ''Escherichia coli'' or ''Bacillus subtilis'' as a chassis for development of MicroMaize:

13 KB (1,962 words) - 02:03, 30 October 2008
Team:Heidelberg/Parts/Characterization
...ebris in the medium. For this characterization ''E. coli'' TOP 10 and ''E. coli'' TOP 10 with transformed cI were used. The added maltose leads to a substa ... Top10 that express cI protein (green curve) as well as untransformed ''E. coli'' (red curve) as control were infected with a bacteriophage λ lacking its

87 KB (12,520 words) - 17:05, 29 October 2008
Team:Heidelberg/Project/Killing II
To proof in principle the feasibility of this system two ''E. coli'' strains were created, one prey strain and one killer strain. The prey str Colicins are toxins produced by ''Escherichia coli'' bacteria, acting against related strains or spices. The toxin is encoded

65 KB (9,654 words) - 17:16, 29 October 2008
Team:University of Sheffield /References
...n Poly-N-acetylglucosamine expression and Biofilm Formation in Escherichia coli .... et al. ( '''2006''') The Cation-Responsive Protein NhaR of ''Excherichia coli'' Activates ''pgaABCD'' Transcription, Required for Production of the Biofi

6 KB (790 words) - 19:32, 28 October 2008
Team:Wisconsin/Project
...rot fungus ''Phanerochaete chrysosporium'', into genetically modified ''E. coli'', capable of producing and exporting the enzyme. Both projects improve cur ...biology. We've designed the following two projects based around using ''E. coli'' to produce biofuels. 

23 KB (3,568 words) - 03:58, 30 October 2008
Team:Bologna/Modeling
E.Coli cells have a system that recovery DNA damage when it occurs. The best studi ... & James J.Collins. Construction of a genetic toggle switch in Escherichia coli, Nature Vol.403 339-342.

20 KB (3,155 words) - 03:29, 30 October 2008
Team:NYMU-Taipei/Project/Attachment
*Design a genetic ''E.coli'' to remove waste through the small intestine epithelium. *When our genetic ''E.coli'' senses the alternation of pH in the small intestine, the adhesion mechani

10 KB (1,506 words) - 19:34, 29 October 2008
Team:BCCS-Bristol/Modeling-Parameters
...ases to some maximum, then decreases as the viscosity increases further. E.coli (strain:KL227 of length: 1.0μm and diameter: 0.5μm) maximum speed Various papers give different speeds for E. coli (most papers provide information on AW405 with a speed of ~20μms-

20 KB (3,132 words) - 15:38, 26 September 2008
Team:Caltech/Project/Phage Pathogen Defense
[[Image:PathogenicEcoli.jpg|thumb|left|Pathogenic ''E. coli''.]] ... this project focuses on engineering a pathogen defense system within ''E. coli''.

17 KB (2,525 words) - 17:00, 25 October 2008
Team:NYMU-Taipei/Project/pH Sensor
...anscription of ''nhaA'', Which Encodes an Na1/H1 Antiporter in Escherichia coli, Is Positively Regulated by '''nhaR''' and Affected by hns] (N. DOVER etc., ...act/196/1/443 Molecular physiology of the Na+/H+ antiporter in Escherichia coli] (E Padan and S Schuldiner, 1994)

5 KB (761 words) - 05:10, 30 October 2008
Team:Paris/Parts
====Promoter of fliL gene from ''E.coli'' flagella==== ...loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.

4 KB (567 words) - 06:48, 30 October 2008
Team:ETH Zurich/Wetlab/Switch Circuit
...G-insensitive mutant, that is under control of the tet repressor. Since E. coli does not produce TetR, a source of constitutively expressed TetR is includ Expression of the lac operon in E. coli is tightly controlled by LacI, a protein, which binds to a repressor

46 KB (7,198 words) - 03:55, 30 October 2008
Team:Harvard/Dailybook/Week1/Chemical and Light
...d||CloDF13||Sm||41 pink colonies and some small yellow ones that may be E. coli ==Growing cultures of E. coli and S1 with BioBrick Parts or Duet Vectors in them==

34 KB (5,237 words) - 18:54, 26 October 2008
Team:KULeuven/Modeling
...438/n7067/full/nature04405.html Synthetic biology: Engineering Escherichia coli to see light] ...e promotor) || 0.025 mRNA/s || see sections on Constitutive Promotors & E. coli transcription Rates

10 KB (1,393 words) - 15:17, 22 July 2008
Team:Bologna/UV radiation/
==E.coli SOS System== [[Image:sopravvivenza.jpg|300px|thumbnail|Fig.1 E.Coli Survival curve|right]]

12 KB (2,040 words) - 09:00, 29 October 2008
Team:ETH Zurich/Project/Motivation
...ed as obligate parasites like Mycoplasma genitalium (580 kbp), Escherichia coli (4.6 Mbp) to Sacharomices cerevisiae (6Mbp). In all cases, the goal is to o ...knockout libraries exist (4), we still are just beginning to understand E. coli as a whole system. Therefore, it is very difficult to decide based on genet

6 KB (907 words) - 09:57, 30 October 2008
Team:ETH Zurich/Wetlab/Chemostat Selection
...roject is to find the minimal genome that is able to sustain a living ''E. coli'' in a specific environmental condition. In the previous section, we have i Our idea is to knockout the thymidylate synthase of the ''E. coli'' strain we use for genome reduction and then add thymidine to the continuo

16 KB (2,353 words) - 04:58, 30 October 2008
Team:Tsinghua/Project
...hosphorylation of CheY, a signaling protein in the chemotaxis pathway of E.coli. Low level of phosphorylation results in counterclockwise rotation of flage ...ly improved a lot. However, the natural ligands concerning chemotaxis in E.coli are limited in several kinds of nutrition, such as carbohydrate and amino a

14 KB (2,003 words) - 04:56, 30 October 2008
Team:Davidson-Missouri Western/Lsr
...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.

5 KB (773 words) - 19:06, 28 October 2008
Lsr
...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.

5 KB (773 words) - 14:04, 29 October 2008
Lsr signaling system
...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.

5 KB (688 words) - 03:52, 30 October 2008
Team:ETH Zurich/Project/Background
...y life with even less genes. The standard laboratory bacterium Escherichia coli is happy with just 4,467 genes (5, 6) and bacteria that live parasitically ...pounds in the nutrient medium and react to changes in concentration (as E. coli does with the lac operon). If the environment grows more adverse, heat shoc

9 KB (1,351 words) - 09:58, 30 October 2008
Team:KULeuven/Model/Kinetic Constants
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” < ...stant upstream site involved in the negative regulation of the Escherichia coli ompF gene.,” Journal of Bacteriology</sp

23 KB (3,482 words) - 12:24, 5 October 2008
Team:ETH Zurich/Modeling/Genome-Scale Model
...nd we use the most updated genome scale model for our working strain (''E. coli'' K12 MG1655) in order to answer the following questions: ...epts, the Flux Balance Analysis theory and in particular the ''iAF1260'' E.Coli Genome Scale Model developed by [http://gcrg.ucsd.edu/|the Palsson's Group

21 KB (3,367 words) - 05:27, 30 October 2008
Team:Bologna/Wetlab
...mage to cell components such as proteins, lipids and principally DNA. In E.coli low concentration of H2O2 (1-3mM) results in SOS gene induction [''Imlay an ...rofiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158:41-64 

20 KB (3,124 words) - 12:57, 4 December 2008
Team:Caltech/Project
... coli'' is capable of killing certain strains of antibiotic resistant ''E. coli'' within six hours. When translated into a probiotic strain such as Nissle ...es and using similar methods to incorporate the non-native phage into ''E. coli''.

8 KB (1,204 words) - 20:49, 29 October 2008
Team:University of Washington/Project
...material, via conjugation, between phylogenetic kingdoms [1]. Escherichia coli, a common laboratory bacterium, is capable of transferring shuttle vectors ...iae are in close proximity and lactose is the primary metabolic source, E. coli cells will construct the conjugative apparatus and transfer the yeast shutt

23 KB (3,437 words) - 04:40, 30 October 2008
Team:TUDelft/Research Proposal
Our aim is to make Escherichia coli act as a thermometer, showing different colors when incubated at differ ...or pathways that lead to color production. When production of FPP in E. coli is achieved, the production of color will be the next goal. To obtain c

17 KB (2,664 words) - 09:53, 30 October 2008
Team:TUDelft/Color overview
...express FPP. There is a downside to this, however, as FPP is toxic to ''E. coli''. This means FPP has to be drained constantly. We will do that by producin ...s been shown that this is an effective strategy to overproduce FPP in ''E. coli'' [[Team:TUDelft/Color_overview#cite_note_3|[3]]]</sp

3 KB (460 words) - 13:59, 29 October 2008
Team:NYMU-Taipei/Project/Time Regulation/Reloxilator
...from the λ phage. The second component uses the HtlB gene from ''E. coli'' attached to the same promoter as the first component. The HtlB gene produ ...eady exists in the strain of ''E. coli'' we're going to use (''Escherichia coli K12 strain MG1655 (tax-id:511145'').

23 KB (2,982 words) - 01:30, 30 October 2008
Team:Illinois/Research articles
===Detection of water-borne E. coli O157 using the integrating waveguide biosensor=== ...r-journal%20papers/1.paper_15-2005-Zhu-BB1.pdf Detection of water-borne E. coli O157 using the integrating waveguide biosensor]

17 KB (2,379 words) - 01:50, 28 October 2008
Team:NYMU-Taipei/Experiments
|It's ''E.coli'' K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test |''E.coli'' K12 MG1655

17 KB (2,381 words) - 08:04, 30 October 2008
Team:University of Chicago/Project
== Synthetic Biology: Engineering E. coli to express Mefp-3,5, and Mgfp-5 proteins == ...p-5 in E. coli, and 4) concomitant expression of Mefp-3,5 and Mgfp-5 in E. coli.

17 KB (2,472 words) - 23:19, 30 May 2008
Reference
2 Construction and Selection of the Novel Recombinant Escherichia cob Strain for Poly(Hydroxybutyrate) Production.HUIMIN YU,JIN YIN,HONGQI '''This literature indicates how PHA sysnthesis is realized in Escherichia coli.'''

3 KB (409 words) - 14:45, 29 October 2008
Team:Alberta NINT/Project
...lating RNA attenuator sequences to generate genetic logic circuits in ''E. coli''...''' == ...NA-mediated transcriptional attenuation mechanism functions in Escherichia coli. J Bacteriol 184:2740-7.

21 KB (3,119 words) - 16:44, 30 March 2009
Team:LCG-UNAM-Mexico/Notebook/2008-June
• If we use a nickel transporter in E. coli we can easily obtain the mutated strain (deleted trasporter). We decided to research how are metals transported by E. coli , each member of the team chose a specific metal: 

16 KB (2,351 words) - 01:14, 29 October 2008
Team:BCCS-Bristol/Notebook
***''E. coli'' MG1655 moved apparently some beads!!! ***The self-made electro competent ''E. coli'' DH5α cells are working

32 KB (4,961 words) - 22:30, 28 October 2008
Team:Purdue/Tools and References
#Web of Science page for "Binding of Photolyase of ''E. coli'' to UV-Damaged DNA" [http://www2.lib.purdue.edu:2149/full_record.do?produc #Sequences of the ''E. coli'' Photolyase gene and protein: [http://www2.lib.purdue.edu:2479/cgi/reprin

6 KB (864 words) - 18:00, 10 July 2008
Team:Caltech
...mally present in the large intestine. Because of its use in research, ''E. coli'' is the most well characterized bacteria to date. Another bacteria, ''Bact ...l bacterial species can cause disease in humans by infecting the gut. ''E. coli'' is commonly associated with food poisoning. ''Salmonela enterica'' is res

8 KB (1,094 words) - 00:53, 30 October 2008
Team:Utah State/Project
recombinant ''E. coli'' and in ''C. necator''. In order to develop an ...tributed to a cost discrepancy of $1.6/kg (Lee SY, 1998). Recombinant ''E. coli'' cells harboring PHB production genes from ''C. necator'' were used in thi

16 KB (2,351 words) - 03:56, 30 October 2008
Team:University of Lethbridge/Project
...we intend to have our riboswitch express the motility protein cheZ in ''E. coli'', thus directing the bacterium towards higher concentrations of our target ...hich responds to this target, a gene involved in motility in ''Escherichia coli'' (''CheZ'') will be switched to an 'on' state (Topp and Gallivan, 2007). '

13 KB (1,787 words) - 04:30, 30 October 2008
Team:TUDelft/Recommendations
...ing our envisioned color pathway by PCR-ing three of the genes out of ''E. coli'', which have been sent to the registry and to a sequencing company. ...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin

6 KB (985 words) - 12:47, 29 October 2008
Team:University of Alberta/Plastic Project:The University of Alberta
We aim to move the human estrogen receptor (hER) into E. coli, which will activate a reporter system in the presence of BPA. When BPA is ... periplasmic and cytoplasmic protein–protein interactions in Escherichia coli: minimal requirements for specific DNA binding and transcriptional activati

14 KB (2,168 words) - 05:32, 30 October 2008
Team:Caltech/Project/Population Variation
... front of LacZ, and then chromosomally integrating the construct into ''E. coli'' so that there is only one copy of the construct in a cell. Our current wo ..., Marcotte EM, and Voigt CA. '''Synthetic biology: engineering Escherichia coli to see light'''. Nature 2005 Nov 24; 438(7067) 441-2.

20 KB (3,252 words) - 18:01, 21 October 2008
Team:TUDelft/Future Work
...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin ...be tested for individual activity, which has not yet been done on the ''E. coli'' genes we provided to the registry. If enzyme activity has been confirmed,

4 KB (599 words) - 22:55, 29 October 2008
Team:Calgary Software/E.Coli CAL516
... scanning electron image of a single E. Coli CAL516 surrounded by other E. Coli bacteria]] ...ythology has been created about confirmed and unconfirmed properties of E. Coli Calgarius. Much of the folklore that surrounds actor [[Team:Calgary_Wetware

978 B (149 words) - 18:03, 25 October 2008
Team:IIT Madras/Project
...ressed sigma-24, sigma-28, sigma-32 and sigma-38 promoters for Escherichia coli. ...truct, and validate a library of sigma-dependent promoters for Escherichia coli, with the following design specifications:

7 KB (1,125 words) - 14:42, 27 October 2008
Team:TUDelft/Color design
...mes from the registry. Of the eight enzymes we will isolate three are ''E. coli'' enzymes (atoB, idi and ispA), while the other five are ''S. cerevisiae'' To PCR the genes out of the ''E. coli'' genome, we've designed primers using the invitrogen website. These primer

5 KB (764 words) - 17:36, 29 October 2008
Team:Slovenia/Notebook/Methods
... DH5α strain was used. After DNA sequences verification E. coli BL21(DE3)pLysS were transformed with specific constructs for the overex ...ogenization Kit (Novagen) to integrate λDE3 prophage into the E. coli host cell chromosome. λDE3 is a recombinant phage carrying a clo

48 KB (6,976 words) - 04:42, 30 October 2008
Team:University of Ottawa/Project
...s are often expressed in simple microorganisms such as bacteria (e.g. ''E. coli'') or yeast. The two most used yeast species are ''Saccharomyces cerevisiae ...urrently limited when compared to the spectrum of parts developed for ''E. coli'', we thought that this would be a novel yeast-based system to expand upon.

22 KB (3,281 words) - 00:50, 30 October 2008
Team:ETH Zurich/Wetlab/Genome Reduction
...tion we discuss about how to randomly delete chromosomal fragments of ''E. coli'' in order to reduce the physical amount of genomic DNA. ...neArt and is supposed to be integrated into the genome of a wild-type ''E. coli'' strain (MG1655). We are planning to use the lambda red recombination syst

12 KB (1,777 words) - 04:34, 30 October 2008
Team:NTU-Singapore/Wetlab/Materials and Equipment
A colony of E. coli W3110 was grown overnight in LB for 16 hours at 37°C . The overnight cultu ...used in all the experiments were LuxS mutants derived from the wild type E coli strain W3110. By knocking out LuxS gene, the cells were unable to synthesiz

6 KB (869 words) - 05:47, 27 October 2008
Team:Bologna/Biosafety
...ral kinds of bacteria and viruses [including canine hepatitis, Escherichia coli, varicella (chicken pox)], as well as some cell cultures and non-infectious ==Genetically modified ''E. coli''==

29 KB (4,412 words) - 02:19, 30 October 2008
Team:Heidelberg/Project/Sensing
...rjik, 2004). For a schematic presentation of the chemotaxis signaling in E.coli see figure 1. ...ain objective of our project is to engineer a so called killer strain of E.coli which would be able to swim by chemotaxis towards a “prey” strain whic

35 KB (5,074 words) - 22:59, 29 October 2008
Team:NTU-Singapore/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

5 KB (768 words) - 14:55, 28 October 2008
Team:KULeuven/Model/Filter
...s of the ribokey/lock and the difference in transcription rates between E. coli's RNA polymerase II and phage T7's polymerase. ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

15 KB (2,344 words) - 23:12, 29 October 2008
Team:NTU-Singapore/Team/Project
...NEERING COLICIN E7 PRODUCTION SYSTEM TO KILL ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 (EHEC) VIA THE DETECTION OF EHEC SYMPTOMS </center> ...se of bacteriocins (i.e. colicin E7) for the killing of the Escherichia Coli O157:H7 enterohemorrhagic strain (EHEC), which causes symptoms such as

4 KB (577 words) - 15:07, 20 October 2008
Team:Hawaii/Project
:Our toolkit was designed for conjugative gene transfer from ''Escherichia coli'' to ''Synechocystis'' to achieve the controlled production and recovery of ... origin of transfer was confirmed by sequencing and will be tested in ''E. coli'' using the construct with ''oriT'' inserted into the ''lacZ'' BioBrick (J3

7 KB (1,011 words) - 03:46, 30 October 2008
Team:Calgary Wetware/Project
...e signal inputs and subsequently initiating transcription of specific ''E. coli''-targeted bacteriocins (''i.e.'' colicins). The presence of AI-1 induces t ...http://partsregistry.org/Part:BBa_J23039 J23039] was transformed into ''E. coli'' thus resulting in Bad Guy #1 cells (i.e. capable of AHL production). To c

10 KB (1,425 words) - 03:10, 30 October 2008
Team:Heidelberg/Project/Killing I
...l fibres, which bind specifically to mannose transporters of ''Escherichia coli''. These receptors are not able to bind stably to structures on other bacte ...modified phages, a GFP cassette for visualization of the phage and an ''E. coli'' oriT (R), which allows the conjugation of the harboring plasmid, provided

55 KB (8,330 words) - 16:49, 29 October 2008
Team:Slovenia/Results/Engineered flagellin vaccine/Engineered bacterial vaccine
...flagellin. We cloned several constructs, which were transformed into E. coli, strain JW1908-1 (+T7 polymerase and bacterial ghost plasmid (BG)). ...e-stained bacteria expressing chimeric flagellin. Flagellin knockout E. coli strain JW1908-1 was transformed with constructs coding for: T7-CF-AK3,

12 KB (1,625 words) - 04:43, 30 October 2008
Team:TUDelft/Temperature design
...ign phase I: Introducing an RNA thermometer into ''Escherichia coli''= ...e RNA thermometers it has been shown that they can be introduced into ''E. coli'', where they still function as a temperature sensitive switch.

9 KB (1,372 words) - 07:59, 29 October 2008
Team:LCG-UNAM-Mexico/Modeling
Escherichia coli nickel efflux pump ...owe JL and Chivers PT (2006) Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function</str

17 KB (2,258 words) - 06:33, 30 October 2008
Team:TUDelft/Temperature design2
.... Note, however, that there will always be an upper bound in that the ''E. coli'' cannot survive temperatures above 40°C, and a lower bound in that the ac ...RNA thermometers for temperature-controlled gene expression in Escherichia coli. ''Nucleic Acids Research'', 1-9, 2008. [http://www.ncbi.nlm.nih.gov/pubmed

20 KB (3,168 words) - 19:33, 29 October 2008
Team:Caltech/Biosafety
*'''Short Term:''' There are no safety concerns beyond that of typical ''E. coli'' lab strains. Considering that 260 mM H2O2 is applie ...K, Schmidt MA, Buer J, and Bruder D. '''Intestinal immunity of Escherichia coli NISSLE 1917: a safe carrier for therapeutic molecules'''. ''FEMS Immunol Me

6 KB (933 words) - 23:57, 29 October 2008
Team:TUDelft/Color analysis
... adding other enzymes. Of the eight enzymes we will isolate three are ''E. coli'' enzymes (atoB, idi and ispA), while the other five are ''S. cerevisiae en ...UDelft/20_August_2008 August 20th]). The next step will be to PCR the ''E. coli'' genes again, with the ideal annealing temperature, using ''Pfx'' polymera

1 KB (234 words) - 13:15, 27 October 2008
Team:Missouri Miners/Project
...have will be isolated from Geobacter sulfurreducens and introduced into E. coli to formulate the most optimal pathway for generating electronmotive force i ...plasmid engineering and the simple fact that Geobacter is anaerobic and E. coli is aerobic. As a team, we will push in the right direction harder than an

6 KB (907 words) - 01:45, 30 October 2008
Team:LCG-UNAM-Mexico/Notebook/2008-July
... Jessica L. and Chivers Peter T., 2006. Nickel homeostasis in Escherichia coli – the rcnR-rcnA efflux pathway and its linkage to NikR function. Mol Mic - Daniela identified the metabolic pathways which involve nickel in E. coli. Here is the <a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=COMPOUND&

22 KB (3,070 words) - 03:27, 29 October 2008
Team:IIT Madras
...>, σ32 and σ38 promoters for Escherichia coli'' ...truct, and validate a library of sigma-dependent promoters for Escherichia coli, with the following design specifications: the promoters must conform to th

3 KB (374 words) - 13:55, 29 September 2008
Team:KULeuven/Model/Memory
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” < ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

26 KB (3,930 words) - 23:24, 29 October 2008
Team:KULeuven/Project/Synthetic Biology
2. Levskaya et al. (2005) Synthetic biology: engineering Escherichia coli to see light. Nature 438:441-442 3. Martin et al. (2003) Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21:796-802

5 KB (768 words) - 14:30, 30 July 2008
Team:University of Sheffield / Late BrSt
...to sense a wide range of pathogens – the coliform bacterias, Escherichia coli, Shigella, Campylobacter, Salmonella enteritidis, Helicobacter sp., Vibrio Two organisms would be modified for quorum sensing purposes, Escherichia coli and Bacillus subtilis. They represent gram negative and gram positive bacte

2 KB (335 words) - 16:51, 31 July 2008
Team:ESBS-Strasbourg/Essential publications
...ichmann.'' Functional determinants of transcription factors in Escherichia coli: protein families and binding sites. (2003) PMID 12547514 ...S. Gardner et al.'' Construction of a genetic toggle switch in Escherichia coli. (2000) PMID 10659857

6 KB (853 words) - 21:20, 29 October 2008
Team:Missouri Miners/Team
*'''''Escherichia coli''''': Chemical and Biological Engineering Image:Ecoli2.jpg| ''Escherichia coli''

4 KB (505 words) - 03:34, 30 October 2008
Team:ETH Zurich/Team/Overview
... Circuitry Exhibiting Toggle Switch or Oscillatory Behavior in Escherichia coli] ...2298.html Multistability in the lactose utilization network of Escherichia coli]

7 KB (1,056 words) - 02:54, 30 October 2008
Team:NTU-Singapore/Wetlab/Protocols
... of interest is the LsrA promoter that is obtained from MG1655 Escherichia Coli strain. Information on the genome of interests is extracted from relevant p

7 KB (996 words) - 05:47, 27 October 2008
Team:Heidelberg/Modeling/Chemotaxis
...deling of Colicin-Induced Killing and Chemotactic Sensing in ''Escherichia Coli''." includes the following information: ...deling of Colicin-Induced Killing and Chemotactic Sensing in ''Escherichia Coli'']]. Further we have made a [[Media:Summary.pdf|summary]] of the complete m

23 KB (3,270 words) - 22:54, 29 October 2008
/BCArchitecture
*See Levskaya et al. ''Synthetic biology: engineering Escherichia coli to see ligh'''. Nature (2005) vol. 438 (7067) pp. 441-2 for more detail *See Rampersaud et al. ''The OmpR protein of Escherichia coli binds to sites in the ompF promoter region in a hierarchical manner determi

4 KB (582 words) - 07:19, 26 October 2008
Team:Melbourne/LinModel
*See Levskaya et al. ''Synthetic biology: engineering Escherichia coli to see ligh'''. Nature (2005) vol. 438 (7067) pp. 441-2 for more detail *See Rampersaud et al. ''The OmpR protein of Escherichia coli binds to sites in the ompF promoter region in a hierarchical manner determi

4 KB (554 words) - 14:17, 29 October 2008
Team:NYMU-Taipei/Project/pLac
... Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens, Current Microbiology 1997] ...mbinatorial transcriptional control of the lactose operon of ''Escherichia coli'', PNAS 2007 (Thomas Kuhlman and et. al)]

2 KB (328 words) - 07:49, 30 October 2008
Team:Guelph/Project
to be electroporated into either probiotic Escherichia coli Nissle 1917 or endophytic ...iotic lactobacilli, corn endophytic ''Klebsiella pneumonii'' 342, or ''E. coli'' Nissle 1917 which is sold in some countries as a probiotic for intestinal

16 KB (2,538 words) - 17:48, 30 May 2018
Team:Caltech/Project/Lactose intolerance
...uman body cannot normally digest. Some bacterial strains, including the E. coli strain ‘Nissle 1917’, can persist in the gut of mice for months. Engine ...ose into glucose and galactose. Since protein secretion is difficult in E. coli, our cells were engineered to lyse in order to release ß-galactosidase. Ho

14 KB (2,041 words) - 01:42, 29 October 2008
Team:LCG-UNAM-Mexico/Parameters
...alue used is the degradation rate of LacY, the lactose permease of E. coli, which is also a transmembran protein.11</div></td> NOTE: The average volume of an E. coli cell is 10-15 liters.

35 KB (4,164 words) - 06:37, 30 October 2008
Team:iHKU/design
...gnaling molecule. We hope the subsequent pattern generated by using E.coli as chassis would be finer and more interesting.

12 KB (1,429 words) - 20:26, 29 October 2008
Team:TU Munchen/Projects/Ear Coli
=Ear Coli (Sound Detector)= Can we make ''E. coli'' detect sound waves?

868 B (115 words) - 15:57, 26 May 2008
Team:University of Chicago/Papers
''[http://www.springerlink.com/content/w8gj1t1j6v540083/fulltext.pdf An E. coli Expression System for the Extracellular Secretion of Barley α-Amylase (pdf ...03477.pdf Enhancement of Mussel Adhesive Protein Production in Escherichia coli by Co-expression of Bacterial Hemoglobin].'' --Jata

7 KB (1,033 words) - 22:07, 26 August 2008
Team:HKUSTers/Project
* '''Strain''': ''E. coli'' BBa_V1001 DH5a, with genotype * Most ordinary ''E. coli'' strains and a variety of pBR322-derived Amp-resistant plasmids can be use

8 KB (1,196 words) - 04:59, 30 October 2008
Team:BCCS-Bristol/Modeling-Batch Simulations
...s relevance to bacterial attachment.] The lipopolysaccharide surface of E. coli usually contains three components; keto deoxy octulonate, core polysacchari ...ontent/99/4/2287.full.pdf+html Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signalling pathway] , measures the alteration in expr

12 KB (1,902 words) - 09:53, 30 September 2008
Team:TUDelft/Temperature results
...ccesful use of this assembly, it is essential that the DB3.1 ''Escherichia coli'' strain is not used as it tolerates the presence of the CcdB gene. Through ...tor - If this happens, cells will not grow after transfection, as the ''E. coli'' strain used should not be CcdB gene tolerant. 

21 KB (3,339 words) - 10:56, 30 October 2008
Team:Groningen/results.html
forward loop could result in the E. coli cells being green even if these cells were not stimulated with HSL. Figure 7.4. Image of E. coli colonies reacting to HSL produced by sender device. Cells were spotted at a

17 KB (2,673 words) - 01:52, 30 October 2008
Team:Edinburgh/Modelling/Kinetic
...kinetic information for the genes in our system, in vivo, as well as in E. coli. Parameter values are currently being fit. ...08). ''Modeling and parameter estimation of the sos response network in E. coli''. Technical report, University of Trento.

3 KB (471 words) - 04:08, 30 October 2008
Team:TUDelft/Color testing
...roof of activity of the pathway would obviously be a colored ''Escherichia coli'' colony. However, to identify individual working enzymes, we'd need specif After we had our first succesful PCR on the genes of ''E. coli'' with ''Taq'' polymerase ([https://2008.igem.org/TUDelft/19_August_2008 La

3 KB (439 words) - 16:37, 29 October 2008
Team:University of Lethbridge/Modeling
...CheY in high enough levels to produce directed movement. We modeled the E. coli strain RP437 in BCT, which is the same strain our group used as the chassis

5 KB (641 words) - 01:00, 30 October 2008
Team:University of Washington
...A via conjugation only if certain conditions are met. In our design, E. coli transfers the genes to metabolize lactose in S. cerevisiae, but

3 KB (439 words) - 23:46, 27 October 2008
Team:KULeuven/Model/Cell Death
...odel/KineticConstants#Constitutive_promoters | Constitutive Promotors & E. coli transcription Rates]] ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

22 KB (3,413 words) - 14:56, 3 October 2008
Team:Groningen/Project
...sions we all agreed that it would be fun to create a system in Escherichia coli which could repeat itself after one initial pulse. ... not want to use a cell-cell signal system which would interfere with ''E. coli's'' own quorum sensing system. We could use these bricks to create a system

5 KB (822 words) - 11:28, 16 September 2008
Team:Paris/Analysis/Design1
... we found and adopted the flagella system of construction in ''Escherichia coli'' which then inspired us further. {{Paris/Toggle|Click here to know more about ''E. coli'' flagella.|Team:Paris/Flagella}}

7 KB (1,165 words) - 03:06, 30 October 2008
Team:Tokyo Aplication project
pressure on gene expression by lac and tac promoters in Escherichia coli.Marine Biotechnology 3:89-92)</div>

7 KB (1,102 words) - 16:19, 26 October 2008
Team:iHKU/protocol
Culture of the E.coli on the 35mm petradish ...h KS, Liu D, Danchin A, Huang JD. Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA seq

63 KB (6,466 words) - 19:25, 29 October 2008
Team:LCG-UNAM-Mexico/Experiments/Design
...., Grass G.”.(2006) "The RcnR (YohLM) system of Escherichia coli: A connection between nickel cobalt and iron homeostasis"Journal of Bacteriology 187 (8), pp. 2912-2916 

19 KB (2,623 words) - 04:08, 30 October 2008
Team:Paris/Flagella
...he genetically defined hierarchy of the flagellar operons in ''Escherichia coli''.]] The construction of ''E. coli'' flagellar system is organized into a complex hierarchy. The different par

4 KB (611 words) - 15:56, 29 October 2008
Team:TUDelft/Color conclusions
...ing our envisioned color pathway by PCR-ing three of the genes out of ''E. coli'', which have been sent to the registry and to a sequencing company.

1 KB (182 words) - 23:06, 29 October 2008
Team:Montreal
...s, which generates oscillations in protein expression within ''Escherichia coli''.

3 KB (461 words) - 05:25, 6 August 2008
Team:KULeuven/Zandbak
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

5 KB (717 words) - 23:45, 28 October 2008
Team:Hawaii/Make Competent E. Coli
[[Category:Escherichia coli]] [[Category:Protocol]]

7 KB (1,158 words) - 23:54, 30 May 2008
Team:TUDelft/Temperature analysis
...RNA thermometers for temperature-controlled gene expression in Escherichia coli. ''Nucleic Acids Research'', 1-9, 2008. [http://www.ncbi.nlm.nih.gov/pubmed

22 KB (3,498 words) - 14:45, 28 October 2008
Team:Purdue/Modeling
...esponse regulation of an excision repair deficient mutant of ''Escherichia coli'' after UV light irradation". [http://www2.lib.purdue.edu:2184/science?_ob

9 KB (1,428 words) - 02:16, 30 October 2008
Team:University of Lethbridge
...e used to control expression of the motility protein cheZ in ''Escherichia coli'' at low concentrations, thus directing the bacterium towards local areas o

6 KB (871 words) - 00:26, 30 October 2008
Team:ETH Zurich/Project
...e(s)”. For convenience reasons we have chosen to work with ''Escherichia coli'' model organism. Currently we are investigating an approach that is outlin

3 KB (523 words) - 13:28, 1 August 2008
Team:NTU-Singapore/Modelling/Parameter
2. Grass G, Otto M, Fricke B, et al., FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress,

9 KB (1,357 words) - 15:14, 28 October 2008
Team:Edinburgh
...007.igem.org/Edinburgh/Yoghurt our 2007 team] in engineering ''Escherichia coli'' to '''produce the vitamin A precursor β-carotene'''. Vitamin A is requir

4 KB (546 words) - 10:48, 7 July 2010
Team:University of Sheffield/Project
...to sense a wide range of pathogens – the coliform bacterias, Escherichia coli, Shigella, Campylobacter, Salmonella enteritidis, Helicobacter sp., Vibrio

3 KB (462 words) - 10:45, 4 July 2008
DNA restriction digest
[[Category:Escherichia coli]]

4 KB (515 words) - 01:22, 17 July 2008
Team:KULeuven/Model/Inverter
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

15 KB (2,288 words) - 23:20, 29 October 2008
Team:KULeuven/Literature
Lon-mediated proteolysis of the Escherichia coli UmuD mutagenesis protein: in vitro degradation and identification of residu

1,004 B (134 words) - 15:51, 3 October 2008
Team:ESBS-Strasbourg/Paper
...JM, Greenfield D, Bustamante C, and Liphardt J. Light-powering Escherichia coli with proteorhodopsin. Proc Natl Acad Sci U S A 2007 Feb 13; 104(7) 2408-12.

3 KB (415 words) - 00:15, 29 October 2008
Team:Tsinghua/Team
...lor: #BDFB01">'''I have my third computer now, it's account is Escherichia coli, and the code is

8 KB (1,001 words) - 04:29, 30 October 2008
Team:Tsinghua/Notebook
1. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. PNAS. 2000, 97(12):6640-6645.

10 KB (1,229 words) - 04:31, 30 October 2008
Team:KULeuven/Model/Output
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

7 KB (1,109 words) - 22:47, 29 October 2008
Team:Paris/Modeling/Bibliography
...Masayori Inouye. EnvZ-OmpR Interaction and Osmoregulation in ''Escherichia coli''. The journal of biochemical chemistry Vol. 277, No. 27, Issue of July 5,

1 KB (189 words) - 14:53, 21 September 2008
Team:Imperial College/Genetic Circuit
...Combinatorial transcriptional control of the lactose operon of Escherichia coli.|PNAS 104 (14)|6043-6048|}}

6 KB (889 words) - 04:03, 27 July 2009
Team:KULeuven/Model/Reset
...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” <

12 KB (1,706 words) - 14:56, 3 October 2008
Team:Chiba/Reference/j
...がわかった。しかし、PAO1とLasI遺伝子を形質転換したE.Coliの上澄み溶液からとれた化合物は、HPLCで同じ保持時間で .../v4/n1/full/msb200824.html FK Balagadde ''et al''.:A synthetic Escherichia coli predator–prey ecosystem.Molecular Systems Biology 4:'''187''',2008] Lu

11 KB (1,081 words) - 11:30, 14 December 2008
Team:BCCS-Bristol/Modeling-GRN
[2] K Otto and T Silhavy. Surface sensing and adhesion of Escherichia coli controlled by the

9 KB (1,344 words) - 14:56, 28 October 2008
Team:NTU-Singapore/Parts/Parameter Estimation using Characterization Results
1. A new logistic model for Escherichia coli growth at constant and dynamic temperatures FUJIKAWA Hiroshi ; KAI Akemi ;

11 KB (1,682 words) - 05:51, 27 October 2008
Team:Paris/Modeling/BOB
...nces H. Arnold, Stephen R. Quake, Lingchong You. ''A Synthetic Escherichia Coli predator prey ecosystem.'' Molecular Systems Biology. 2008.

24 KB (3,732 words) - 04:46, 30 October 2008
Team:Heidelberg/Human Practice/Phips the Phage/General Backround
...ell known to scientists are '''''Escherichia coli''''' (short version: ''E.coli'') bacteria. They are organisms naturally living in our lower intestine - a ... grow and their genetics are relatively simple and easy to manipulate, ''E.coli'' is one of the best-studied model organisms in biotechnology.

47 KB (7,271 words) - 03:19, 30 April 2016
Team:Chiba/Reference
.../v4/n1/full/msb200824.html FK Balagadde ''et al''.:A synthetic Escherichia coli predator–prey ecosystem.Molecular Systems Biology 4:'''187''',2008]

8 KB (1,072 words) - 01:21, 30 October 2008
Team:NTU-Singapore/Wetlab
...r these parts were extracted from their wild bacteria strains (Escherichia Coli MG1655, BW and W3110) and modified to become standard biobrick modules. The

4 KB (647 words) - 05:46, 27 October 2008
Team:Heidelberg/Notebook/seminar
| Kathrin || F. K. Balagaddké ''et al.'', A synthetic ''Escherichia coli'' predator-prey ecosystem, ''Molecular Systems Biology'', Vol. 4, pp. 187-1

24 KB (3,291 words) - 15:56, 29 October 2008
Team:Slovenia/Background/The problem
opposite to LPS and flagellin of Escherichia coli), which results in no activation of the immune system cells.

27 KB (3,548 words) - 03:59, 30 October 2008
Team:Mexico-UNAM-IPN/Horizontal-Transfer/30 September 2008
...g>'''JM101'''</big>: In blue the corresponding vials to this ''Escherichia coli'' strain.

984 B (147 words) - 03:42, 28 October 2008
Team:Mexico-UNAM-IPN/Horizontal-Transfer/29 September 2008
...g>'''JM101'''</big>: In blue the corresponding vials to this ''Escherichia coli''strain.

830 B (131 words) - 21:56, 27 October 2008
Team:Mexico-UNAM-IPN/Horizontal-Transfer/8 September 2008
For each vial 15ml of LB media and culture the strains of ''Escherichia coli'' in glycerol stock.

357 B (50 words) - 03:59, 28 October 2008
Team:BrownTwo/Protocols/compcells
[[Category:Escherichia coli]]

5 KB (756 words) - 08:51, 28 October 2008
Team:Imperial College/Light
...enfield, D.; Bustamante, C. & Liphardt, J.|2007|Light-powering Escherichia coli with proteorhodopsin|Proc Natl Acad Sci 104(7)|2408-12||}} ([http://www.ncb

6 KB (888 words) - 18:48, 29 October 2008
Team:Imperial College/Genetic Circuit Details
...Combinatorial transcriptional control of the lactose operon of Escherichia coli.|PNAS 104 (14)|6043-6048|}}

4 KB (640 words) - 02:50, 30 October 2008
Team:Rice University/STRATEGY
... as a functional protein in ''Saccharomyces cerevisiae'' and ''Escherichia coli''. While the ''p''-coumaric acid produced by TAL will serve as a substrate

7 KB (1,032 words) - 05:09, 30 October 2008
Team:Harvard/References
...Independent and tight regulation of transcriptional units in ''Escherichia coli'' via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. ''Nucleic

2 KB (291 words) - 02:59, 30 October 2008
Team:Paris/Bibliography
...nces H. Arnold, Stephen R. Quake, Lingchong You. ''A Synthetic Escherichia Coli predator prey ecosystem.'' Molecular Systems Biology. 2008.

1 KB (188 words) - 14:18, 29 October 2008
Team:NYMU-Taipei/Project/Phosphate/Simulations/Phosphate
...genetically separable inorganic phosphate transport systems in Escherichia coli, JB 1980 (G R Willsky and M H Malamy)]

1 KB (215 words) - 09:16, 30 October 2008