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• Jamboree/Project Abstract/Team Abstracts
  ...to-Builders'', we investigate the possibility of using large numbers of E. coli to perform a task too great for any individual cell. Specifically, this in ''Ecoli.PROM: an Erasable and Programmable Genetic M­­emory with E. coli''
  76 KB (11,186 words) - 15:27, 6 November 2008
• Team:NYMU-Taipei/Project/Phosphate
  * Enhance and regulate the phosphate absorption of ''E.coli'' to balance phosphate concentration to a normal level in patients with kid === Phosphate Metabolism and Regulation in ''E.coli'' ===
  7 KB (967 words) - 05:14, 30 October 2008
• Team:University of Sheffield /Project
  ... cholera pathogens expression of the receptor protein would transform an E.coli into a biosensor. The most straightforward, which does not mean the easiest ...nal conveyer domain of BarA which is a protein present on the surface of E.coli cell and that passes signal downstream to DNA.
  22 KB (3,311 words) - 01:07, 29 October 2008
• Team:Edinburgh/Plan
  == The chassis: ''E. coli'' vs. ''B. subtilis'' == We were initially torn between using ''Escherichia coli'' or ''Bacillus subtilis'' as a chassis for development of MicroMaize:
  13 KB (1,962 words) - 02:03, 30 October 2008
• Team:Heidelberg/Parts/Characterization
  ...ebris in the medium. For this characterization ''E. coli'' TOP 10 and ''E. coli'' TOP 10 with transformed cI were used. The added maltose leads to a substa ... Top10 that express cI protein (green curve) as well as untransformed ''E. coli'' (red curve) as control were infected with a bacteriophage λ lacking its
  87 KB (12,520 words) - 17:05, 29 October 2008
• Team:Heidelberg/Project/Killing II
  To proof in principle the feasibility of this system two ''E. coli'' strains were created, one prey strain and one killer strain. The prey str Colicins are toxins produced by ''Escherichia coli'' bacteria, acting against related strains or spices. The toxin is encoded
  65 KB (9,654 words) - 17:16, 29 October 2008
• Team:University of Sheffield /References
  ...n Poly-N-acetylglucosamine expression and Biofilm Formation in Escherichia coli .... et al. ( '''2006''') The Cation-Responsive Protein NhaR of ''Excherichia coli'' Activates ''pgaABCD'' Transcription, Required for Production of the Biofi
  6 KB (790 words) - 19:32, 28 October 2008
• Team:Wisconsin/Project
  ...rot fungus ''Phanerochaete chrysosporium'', into genetically modified ''E. coli'', capable of producing and exporting the enzyme. Both projects improve cur ...biology. We've designed the following two projects based around using ''E. coli'' to produce biofuels. <br><br>
  23 KB (3,568 words) - 03:58, 30 October 2008
• Team:Bologna/Modeling
  E.Coli cells have a system that recovery DNA damage when it occurs. The best studi ... & James J.Collins. Construction of a genetic toggle switch in Escherichia coli, Nature Vol.403 339-342.
  20 KB (3,155 words) - 03:29, 30 October 2008
• Team:NYMU-Taipei/Project/Attachment
  *Design a genetic ''E.coli'' to remove waste through the small intestine epithelium. *When our genetic ''E.coli'' senses the alternation of pH in the small intestine, the adhesion mechani
  10 KB (1,506 words) - 19:34, 29 October 2008
• Team:BCCS-Bristol/Modeling-Parameters
  ...ases to some maximum, then decreases as the viscosity increases further. E.coli (strain:KL227 of length: 1.0&#956;m and diameter: 0.5&#956;m) maximum speed Various papers give different speeds for E. coli (most papers provide information on AW405 with a speed of ~20&#956;ms<sup>-
  20 KB (3,132 words) - 15:38, 26 September 2008
• Team:Caltech/Project/Phage Pathogen Defense
  [[Image:PathogenicEcoli.jpg|thumb|left|Pathogenic ''E. coli''.]] ... this project focuses on engineering a pathogen defense system within ''E. coli''.
  17 KB (2,525 words) - 17:00, 25 October 2008
• Team:NYMU-Taipei/Project/pH Sensor
  ...anscription of ''nhaA'', Which Encodes an Na1/H1 Antiporter in Escherichia coli, Is Positively Regulated by '''nhaR''' and Affected by hns] (N. DOVER etc., ...act/196/1/443 Molecular physiology of the Na+/H+ antiporter in Escherichia coli] (E Padan and S Schuldiner, 1994)
  5 KB (761 words) - 05:10, 30 October 2008
• Team:Paris/Parts
  ====Promoter of fliL gene from ''E.coli'' flagella==== ...loop with a SUM input function prolongs flagella expression in Escherichia coli. Mol Syst Biol. 2005;1:2005.0006. Epub 2005 Mar 29.
  4 KB (567 words) - 06:48, 30 October 2008
• Team:ETH Zurich/Wetlab/Switch Circuit
  ...G-insensitive mutant, that is under control of the tet repressor. Since E. coli does not produce TetR, a source of constitutively expressed TetR is includ Expression of the lac operon in E. coli is tightly controlled by LacI, a protein, which binds to a repressor
  46 KB (7,198 words) - 03:55, 30 October 2008
• Team:Harvard/Dailybook/Week1/Chemical and Light
  ...d||CloDF13||Sm||41 pink colonies and some small yellow ones that may be E. coli ==Growing cultures of E. coli and S1 with BioBrick Parts or Duet Vectors in them==
  34 KB (5,237 words) - 18:54, 26 October 2008
• Team:KULeuven/Modeling
  ...438/n7067/full/nature04405.html Synthetic biology: Engineering Escherichia coli to see light] ...e promotor) || 0.025 mRNA/s || see sections on Constitutive Promotors & E. coli transcription Rates
  10 KB (1,393 words) - 15:17, 22 July 2008
• Team:Bologna/UV radiation/
  ==E.coli SOS System== [[Image:sopravvivenza.jpg|300px|thumbnail|Fig.1 E.Coli Survival curve|right]]
  12 KB (2,040 words) - 09:00, 29 October 2008
• Team:ETH Zurich/Project/Motivation
  ...ed as obligate parasites like Mycoplasma genitalium (580 kbp), Escherichia coli (4.6 Mbp) to Sacharomices cerevisiae (6Mbp). In all cases, the goal is to o ...knockout libraries exist (4), we still are just beginning to understand E. coli as a whole system. Therefore, it is very difficult to decide based on genet
  6 KB (907 words) - 09:57, 30 October 2008
• Team:ETH Zurich/Wetlab/Chemostat Selection
  ...roject is to find the minimal genome that is able to sustain a living ''E. coli'' in a specific environmental condition. In the previous section, we have i Our idea is to knockout the thymidylate synthase of the ''E. coli'' strain we use for genome reduction and then add thymidine to the continuo
  16 KB (2,353 words) - 04:58, 30 October 2008
• Team:Tsinghua/Project
  ...hosphorylation of CheY, a signaling protein in the chemotaxis pathway of E.coli. Low level of phosphorylation results in counterclockwise rotation of flage ...ly improved a lot. However, the natural ligands concerning chemotaxis in E.coli are limited in several kinds of nutrition, such as carbohydrate and amino a
  14 KB (2,003 words) - 04:56, 30 October 2008
• Team:Davidson-Missouri Western/Lsr
  ...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.
  5 KB (773 words) - 19:06, 28 October 2008
• Lsr
  ...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.
  5 KB (773 words) - 14:04, 29 October 2008
• Lsr signaling system
  ...om DPD. [http://iai.asm.org/cgi/reprint/73/6/3197.pdf AI-2 is R-THMF in E. coli] lsrA,B,C,D,F,G,R,K: Escherichia coli HS, SMS-3-5, str. K12 substr. MG1655, and substr. DH10B.
  5 KB (688 words) - 03:52, 30 October 2008
• Team:ETH Zurich/Project/Background
  ...y life with even less genes. The standard laboratory bacterium Escherichia coli is happy with just 4,467 genes (5, 6) and bacteria that live parasitically ...pounds in the nutrient medium and react to changes in concentration (as E. coli does with the lac operon). If the environment grows more adverse, heat shoc
  9 KB (1,351 words) - 09:58, 30 October 2008
• Team:KULeuven/Model/Kinetic Constants
  ...tein et al., “Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays,” < ...stant upstream site involved in the negative regulation of the Escherichia coli ompF gene.,” <span style="font-style:italic;">Journal of Bacteriology</sp
  23 KB (3,482 words) - 12:24, 5 October 2008
• Team:ETH Zurich/Modeling/Genome-Scale Model
  ...nd we use the most updated genome scale model for our working strain (''E. coli'' K12 MG1655) in order to answer the following questions: ...epts, the Flux Balance Analysis theory and in particular the ''iAF1260'' E.Coli Genome Scale Model developed by [http://gcrg.ucsd.edu/|the Palsson's Group
  21 KB (3,367 words) - 05:27, 30 October 2008
• Team:Bologna/Wetlab
  ...mage to cell components such as proteins, lipids and principally DNA. In E.coli low concentration of H2O2 (1-3mM) results in SOS gene induction [''Imlay an ...rofiles following UV exposure in wild-type and SOS-deficient Escherichia coli. Genetics 158:41-64<br>
  20 KB (3,124 words) - 12:57, 4 December 2008
• Team:Caltech/Project
  ... coli'' is capable of killing certain strains of antibiotic resistant ''E. coli'' within six hours. When translated into a probiotic strain such as Nissle ...es and using similar methods to incorporate the non-native phage into ''E. coli''.
  8 KB (1,204 words) - 20:49, 29 October 2008
• Team:University of Washington/Project
  ...material, via conjugation, between phylogenetic kingdoms [1]. Escherichia coli, a common laboratory bacterium, is capable of transferring shuttle vectors ...iae are in close proximity and lactose is the primary metabolic source, E. coli cells will construct the conjugative apparatus and transfer the yeast shutt
  23 KB (3,437 words) - 04:40, 30 October 2008
• Team:TUDelft/Research Proposal
  Our aim is to make <i>Escherichia coli</i> act as a thermometer, showing different colors when incubated at differ ...or pathways that lead to color production. When production of FPP in <i>E. coli</i> is achieved, the production of color will be the next goal. To obtain c
  17 KB (2,664 words) - 09:53, 30 October 2008
• Team:TUDelft/Color overview
  ...express FPP. There is a downside to this, however, as FPP is toxic to ''E. coli''. This means FPP has to be drained constantly. We will do that by producin ...s been shown that this is an effective strategy to overproduce FPP in ''E. coli'' <span id="cite_ref_3">[[Team:TUDelft/Color_overview#cite_note_3|[3]]]</sp
  3 KB (460 words) - 13:59, 29 October 2008
• Team:NYMU-Taipei/Project/Time Regulation/Reloxilator
  ...from the &lambda; phage. The second component uses the HtlB gene from ''E. coli'' attached to the same promoter as the first component. The HtlB gene produ ...eady exists in the strain of ''E. coli'' we're going to use (''Escherichia coli K12 strain MG1655 (tax-id:511145'').
  23 KB (2,982 words) - 01:30, 30 October 2008
• Team:Illinois/Research articles
  ===Detection of water-borne E. coli O157 using the integrating waveguide biosensor=== ...r-journal%20papers/1.paper_15-2005-Zhu-BB1.pdf Detection of water-borne E. coli O157 using the integrating waveguide biosensor]
  17 KB (2,379 words) - 01:50, 28 October 2008
• Team:NYMU-Taipei/Experiments
  |It's ''E.coli'' K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test |''E.coli'' K12 MG1655
  17 KB (2,381 words) - 08:04, 30 October 2008
• Team:University of Chicago/Project
  == Synthetic Biology: Engineering E. coli to express Mefp-3,5, and Mgfp-5 proteins == ...p-5 in E. coli, and 4) concomitant expression of Mefp-3,5 and Mgfp-5 in E. coli.
  17 KB (2,472 words) - 23:19, 30 May 2008
• Reference
  2 Construction and Selection of the Novel Recombinant Escherichia cob Strain for Poly(Hydroxybutyrate) Production.HUIMIN YU,JIN YIN,HONGQI '''This literature indicates how PHA sysnthesis is realized in Escherichia coli.'''
  3 KB (409 words) - 14:45, 29 October 2008
• Team:Alberta NINT/Project
  ...lating RNA attenuator sequences to generate genetic logic circuits in ''E. coli''...''' == ...NA-mediated transcriptional attenuation mechanism functions in Escherichia coli. J Bacteriol 184:2740-7.
  21 KB (3,119 words) - 16:44, 30 March 2009
• Team:LCG-UNAM-Mexico/Notebook/2008-June
  • If we use a nickel transporter in <i>E. coli</i> we can easily obtain the mutated strain (deleted trasporter). <br /> <p>We decided to research how are metals transported by <i> E. coli </i>, each member of the team chose a specific metal: </p>
  16 KB (2,351 words) - 01:14, 29 October 2008
• Team:BCCS-Bristol/Notebook
  ***''E. coli'' MG1655 moved apparently some beads!!! ***The self-made electro competent ''E. coli'' DH5α cells are working
  32 KB (4,961 words) - 22:30, 28 October 2008
• Team:Purdue/Tools and References
  #Web of Science page for "Binding of Photolyase of ''E. coli'' to UV-Damaged DNA" [http://www2.lib.purdue.edu:2149/full_record.do?produc #Sequences of the ''E. coli'' Photolyase gene and protein: [http://www2.lib.purdue.edu:2479/cgi/reprin
  6 KB (864 words) - 18:00, 10 July 2008
• Team:Caltech
  ...mally present in the large intestine. Because of its use in research, ''E. coli'' is the most well characterized bacteria to date. Another bacteria, ''Bact ...l bacterial species can cause disease in humans by infecting the gut. ''E. coli'' is commonly associated with food poisoning. ''Salmonela enterica'' is res
  8 KB (1,094 words) - 00:53, 30 October 2008
• Team:Utah State/Project
  recombinant ''E. coli'' and in ''C. necator''. In order to develop an ...tributed to a cost discrepancy of $1.6/kg (Lee SY, 1998). Recombinant ''E. coli'' cells harboring PHB production genes from ''C. necator'' were used in thi
  16 KB (2,351 words) - 03:56, 30 October 2008
• Team:University of Lethbridge/Project
  ...we intend to have our riboswitch express the motility protein cheZ in ''E. coli'', thus directing the bacterium towards higher concentrations of our target ...hich responds to this target, a gene involved in motility in ''Escherichia coli'' (''CheZ'') will be switched to an 'on' state (Topp and Gallivan, 2007). '
  13 KB (1,787 words) - 04:30, 30 October 2008
• Team:TUDelft/Recommendations
  ...ing our envisioned color pathway by PCR-ing three of the genes out of ''E. coli'', which have been sent to the registry and to a sequencing company. ...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin
  6 KB (985 words) - 12:47, 29 October 2008
• Team:University of Alberta/Plastic Project:The University of Alberta
  We aim to move the human estrogen receptor (hER) into E. coli, which will activate a reporter system in the presence of BPA. When BPA is ... periplasmic and cytoplasmic protein–protein interactions in Escherichia coli: minimal requirements for specific DNA binding and transcriptional activati
  14 KB (2,168 words) - 05:32, 30 October 2008
• Team:Caltech/Project/Population Variation
  ... front of LacZ, and then chromosomally integrating the construct into ''E. coli'' so that there is only one copy of the construct in a cell. Our current wo ..., Marcotte EM, and Voigt CA. '''Synthetic biology: engineering Escherichia coli to see light'''. Nature 2005 Nov 24; 438(7067) 441-2.
  20 KB (3,252 words) - 18:01, 21 October 2008
• Team:TUDelft/Future Work
  ...uggest using a different enzyme. This would have to be a non ''Escherichia coli'' enzyme which is easily measured, preferably in whole cells. It is temptin ...be tested for individual activity, which has not yet been done on the ''E. coli'' genes we provided to the registry. If enzyme activity has been confirmed,
  4 KB (599 words) - 22:55, 29 October 2008
• Team:Calgary Software/E.Coli CAL516
  ... scanning electron image of a single E. Coli CAL516 surrounded by other E. Coli bacteria]] ...ythology has been created about confirmed and unconfirmed properties of E. Coli Calgarius. Much of the folklore that surrounds actor [[Team:Calgary_Wetware
  978 B (149 words) - 18:03, 25 October 2008
• Team:IIT Madras/Project
  ...ressed sigma-24, sigma-28, sigma-32 and sigma-38 promoters for Escherichia coli. ...truct, and validate a library of sigma-dependent promoters for Escherichia coli, with the following design specifications:
  7 KB (1,125 words) - 14:42, 27 October 2008