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  • Team:Chiba/jk/β/weaks 1-2
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/PCR|PCR]]'''
    43 KB (6,909 words) - 07:07, 20 September 2008
  • Team:Chiba/jk/β/week 2
    >[[Team:Chiba/protocol/j|Protocols]] :(22/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    22 KB (3,468 words) - 12:11, 23 October 2008
  • Team:Chiba/notebook/input/august
    '''[[Team:Chiba/protocol/PCR|PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    17 KB (2,289 words) - 05:02, 24 October 2008
  • Team:Chiba/jk/β/week 3
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    26 KB (4,447 words) - 04:05, 24 October 2008
  • Team:Chiba/Calendar-Home/30 August 2008
    '''[[Team:Chiba/protocol/digestion|Digestion]]''' -->30μl'''[[Team:Chiba/protocol/ligation/gelex|Gel extraction]]'''
    11 KB (1,784 words) - 23:13, 29 October 2008
  • Team:Chiba/protocol/j
    ===[[Team:Chiba/protocol/transformation/j|Z コンピ]] === ===[[Team:Chiba/protocol/DNA_Purification/sigma/j|シグマプレップ]]===
    4 KB (188 words) - 05:02, 27 June 2009
  • Team:Cambridge/Bacillus/Lab Work
    '''Protocol''' *Add Disks in antibiotic solution for 5 mins [ Protocol to sterilize tweezers : Wipe with Kimwipes, Ethanol, Flame ]
    65 KB (9,746 words) - 03:56, 30 October 2008
  • Team:Chiba/protocol
    ===[[Team:Chiba/protocol/transformation|Zymo comp.]] === ===[[Team:Chiba/protocol/transformation|Electro poration]]===
    1 KB (181 words) - 12:46, 16 December 2008
  • Team:iHKU/protocol
    <td><a href="/Team:iHKU/protocol"><img src="/wiki/images/f/f2/Button_protocol.jpg" width="200" height="25" / <li> PROTOCOL-1 <a href="#p1">Pattern formation</a></li>
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:Chiba/jk/β/week 1
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    22 KB (3,633 words) - 12:34, 30 September 2008
  • Team:Chiba/jk/β/week 5
    >[[Team:Chiba/protocol/j|Protocols]] :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    6 KB (804 words) - 13:20, 1 October 2008
  • GenProtocols
    This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from ==Ligation protocol, using Roche Rapid Ligation Kit==
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Team:BrownTwo/Notebook
    ...or about 4.5 hours since other obligations prevented us from finishing the protocol. Normally, we would have plated the transformations immediately after they <p>Gel Mixture Protocol for 1% Agarose gel:</p>
    226 KB (32,672 words) - 04:53, 29 October 2008
  • Team:Paris/August 8
    ==== '''Protocol''' ==== ==== Protocol : ====
    18 KB (2,438 words) - 13:22, 15 August 2008
  • Team:Chiba/jk/β/weaks 3-4
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/digestion|Digestion]]'''
    13 KB (2,259 words) - 07:03, 20 September 2008
  • Team:Harvard/GenProtocols
    ==Ligation protocol, using Roche Rapid Ligation Kit== ===Brief Protocol===
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Paris/August 15
    ==='''Protocol'''=== ...ly competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]].
    9 KB (1,174 words) - 18:06, 4 September 2008
  • Team:Chiba/Calendar-Home/1 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' '''[[Team:Chiba/protocol/transformation|Transformation]]'''(Double Transformation)
    8 KB (1,254 words) - 01:42, 30 October 2008
  • Team:Chiba/jk/γ/week 2
    [[Team:Chiba/protocol/transformation|Transformation]] [[Team:Chiba/protocol/digestion|Digestion]]
    9 KB (1,396 words) - 05:50, 24 October 2008
  • Team:Chiba/Calendar-Home/26 August 2008
    :(8/25)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
    7 KB (1,128 words) - 01:04, 30 October 2008
  • Team:Calgary Wetware/Protocols
    ...I wrote up a procedure for diluting primers. As an added bonus, contains a protocol for PCR purification! [[Team:Calgary_Wetware/Protocols/Diluting|Check it ou <li><a href="#transformation" title="Bacterial Transformation Protocol"> Bacterial Transformation </a></li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • Team:TUDelft/Protocols
    ...We did make a stock of chemically competent DB3.1 cells with the following protocol (found on OpenWetWare). We found that these cells were indeed very competen ''Protocol found on OpenWetWare''
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:KULeuven/Data/Other Parts
    ...s can be tested (the construct that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    14 KB (2,307 words) - 23:28, 29 October 2008
  • Team:Chiba/jk/β/week 4
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (714 words) - 12:52, 30 September 2008
  • Team:Paris/August 6
    ==== Protocol ==== ====Protocol====
    8 KB (1,222 words) - 14:06, 13 August 2008
  • Team:Illinois/Bimolecular Fluorescence Biosensor Notebook
    ** PCR protocol as in paper for both N terminal * Gel Extraction Protocol
    14 KB (2,108 words) - 18:50, 29 October 2008
  • Team:Chiba/Calendar-Home/28 August 2008
    '''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    5 KB (757 words) - 03:14, 30 October 2008
  • July
    -&gt; protocol 07-10-2008 <br> we repeated step 5 and 6 of the protocol.<br>
    22 KB (3,483 words) - 02:40, 30 October 2008
  • Team:Imperial College/Light Inducible
    The aim of this protocol is to characterise the light induced expression of GFPmut3b under the contr ======Protocol======
    6 KB (938 words) - 17:44, 28 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...at6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry o
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:University of Lethbridge/Notebook/Project1August
    ...ok & Russell, (2001) ''Molecular Cloning: A Laboratory Manual 3rd Edition. Protocol 25'' '''Vol. 1; 1.117'''): Protocol:
    13 KB (1,866 words) - 02:06, 30 October 2008
  • Team:Paris/August 27
    [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    5 KB (688 words) - 19:16, 9 September 2008
  • Team:KULeuven/Data/Other Components
    ... can be tested (the constructs that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    9 KB (1,463 words) - 20:13, 29 October 2008
  • Team:Hawaii/Initial PCC6803 Transformation
    == Protocol by Arizona State University == == Protocol by Shao, ''et al'' ==
    4 KB (628 words) - 06:54, 27 June 2008
  • Team:Hawaii/Initial E. Coli Transformation
    == Protocol by Invitrogen == == Protocol from SC Lab ==
    6 KB (893 words) - 02:31, 2 August 2008
  • Team:Paris/August 7
    *Concerning '''pflhB, pflgA and pflgB''', the protocol seems to be '''very operational''': we always have great results ! *Standart protocol except the last step. Instead of eluting with water, we used 30 µL of BE b
    10 KB (1,412 words) - 12:42, 13 August 2008
  • Team:Illinois/Antibody GPCR Fusion Notebook
    **Protocol from Wiley's ''Current Protocols in Molecular Biology'' ** Result: Failed to finish protocol
    13 KB (1,969 words) - 02:55, 30 October 2008
  • Team:Paris/August 22
    [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]]
    7 KB (891 words) - 00:54, 21 September 2008
  • Team:Chiba/Calendar-Home/29 August 2008
    :--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (803 words) - 20:28, 29 October 2008
  • Team:Paris/August 21
    ==Protocol== [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    7 KB (836 words) - 14:29, 6 September 2008
  • Team:Paris/August 26
    ==Protocol== '''Protocol'''
    11 KB (1,582 words) - 17:59, 11 September 2008
  • Team:Chiba/jk/γ/week 3
    [[Team:Chiba/protocol/digestion|Digestion]] [[Team:Chiba/protocol/ligation/ligation|Ligation]]
    6 KB (946 words) - 06:30, 24 October 2008
  • Team:Chiba/jk/γ/week 4
    [[Team:Chiba/protocol/digestion|Digestion]] -->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
    6 KB (907 words) - 06:52, 24 October 2008
  • Team:TUDelft/Temperature results
    ...ure_results#Setting_up_luciferase_measurements|Obtaining a correct working protocol to measure luminescence]] ...onstruction of all devices the 3A assembly strategy was chosen. A complete protocol of this strategy can be found on [http://openwetware.org/wiki/Synthetic_Bio
    21 KB (3,339 words) - 10:56, 30 October 2008
  • Team:Chiba/Calendar-Home/19 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''<br>
    3 KB (476 words) - 00:20, 30 October 2008
  • Team:Imperial College/Transformation Results
    ...m:Imperial_College/Transformation Click this link for full Transformation protocol 1 '''] '''Brief Description of Protocol'''<br>
    4 KB (559 words) - 01:59, 30 October 2008
  • Team:Michigan/Notebook/PCRProtocol
    == <font color=royalblue size=5> PCR Standard Protocol </font> == ... machines may also require different mixtures to run properly. This is the protocol specified for our machine, the PCR Express.
    2 KB (365 words) - 03:18, 30 October 2008
  • Team:Cambridge/Signalling/Lab Work
    * found protocol for Bacillus subtilis transformation ** Miniprep I746001 and I746101 pellets (plasmid mniprep kit) : same protocol but 60μL of Zyppy Glution Buffer in step 8
    36 KB (5,743 words) - 04:15, 30 October 2008
  • Extract DNA (Maxiprep or Midiprep)
    ==Midiprep/ Maxiprep Growth Protocol== ==Midiprep Protocol (QIAGEN kit)==
    6 KB (1,068 words) - 01:00, 30 October 2008
  • Team:Hawaii/Initial Synth. Oligo Assembly
    ==Protocol== ...e.org/wiki/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR her protocol on OpenWetWare]. No visible difference was detected between the three annea
    19 KB (3,019 words) - 16:40, 25 July 2008

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