Team:Beijing Normal/Notebook

From 2008.igem.org

Home The Team The Project Parts Submitted to the Registry Notebook

Dynamics

  • == Communications ==
    • 17th June: Attend the Asian Workshop meeting.
    • 17th July: Meet up with Tsinghua Team, and learn fron their experiences.
    • 25th July: With Invitrogen.Co. Ltd and Tsinghua team to sign up a contract.
    • 27th July: Have a discussion with Tsinghua team about the transformation efficiency.

We generalize some key words from the discussion:

1. Make sure the concentration of the plasmids from the biobrick is enough for transformation.

2. Keep a low temprature when enzyme digestion is performed

3. The transformation product are always incubated for more than 1h.


    • 9th September: We and Tsinghua team get together in our lab. We have a happy time together and share a lot.
    • 10th September: We provide Tsinghua Team with the cells harboring pSB1AC3 plasmids. We did the the tramsformation for them. Because they found their transformation effeciency is relatively low.
    • 17th September: Tsinghua Team told us that the vector that we provided proved to work well. We were all pretty happy about this positive result.
    • 3th October: We and Chiba Team had a happy conversation via googletalk today. We share all the success and frustrations in the process. And we exchange our opinion about promotor function measurement. They told us that they have used BBa_T9002 because their project uses quorum sensing and the part(T9002) was GFP producer controlled by 3OC6HSL Receiver Device.And their result is pretty satisfying.
    • 5th October: We share the experience of measurment with Tokyo Tech University Team. They tell us to use BBa_I13522 (http://partsregistry.org/Part:BBa_I13522) as a positive control when we measure the function of promotors.
    • 9th October: We test the founction of Part BBa_I719015. The result indicates that it works well. The cells (BL21(DE3) as host) turn green when induced by IPTG.
    • 15th October: Our T-shirt is designed today. It is very pretty. See image below.
    • 19th, 20th October: We have a good discussion with USTC team in Hefei and they give us several important suggestions on experiments.

T-shirt.jpg

T-shirt1.jpg

Our logo is designed finally. It is a visual field through a microscope. You could see us in this microscope.

Our logo.jpg

    • Our iGem is coming to the end. But our projet will continue.

Of course, we encountered many obstacles and difficulties such as financial problems, cooperation and communication problems of teammates, spirit pressure, time management and so on. But all the hardship turned out to be a precious experience that helped us grow up. Anyway, iGem is one of our most precious experiences and we will never forget these days and night in the lab and meeting room, all the laughs and tears. We have learned a lot in this project except the attending the competition itself.



July
MTWTFSS
  [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/1_July_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/2_July_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/3_July_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/6_July_2008&action=edit 6]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/7_July_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/8_July_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/9_July_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/10_July_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/11_July_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_July_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_July_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_July_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_July_2008&action=edit 17] [http://2008.igem.org/Beijing_Normal/18_July_2008 18] [http://2008.igem.org/Beijing_Normal/19_July_2008 19] [http://2008.igem.org/Beijing_Normal/20_July_2008 20]
[http://2008.igem.org/Beijing_Normal/21_July_2008 21] [http://2008.igem.org/Beijing_Normal/22_July_2008 22] [http://2008.igem.org/Beijing_Normal/23_July_2008 23] [http://2008.igem.org/Beijing_Normal/24_July_2008 24] [http://2008.igem.org/Beijing_Normal/25_July_2008 25] [http://2008.igem.org/Beijing_Normal/26_July_2008 26] [http://2008.igem.org/Beijing_Normal/27_July_2008 27]
[http://2008.igem.org/Beijing_Normal/28_July_2008 28] [http://2008.igem.org/Beijing_Normal/29_July_2008 29] [http://2008.igem.org/Beijing_Normal/30_July_2008 30] [http://2008.igem.org/Beijing_Normal/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Beijing_Normal/1_August_2008 1] [http://2008.igem.org/Beijing_Normal/2_August_2008 2] [http://2008.igem.org/Beijing_Normal/3_August_2008 3]
[http://2008.igem.org/Beijing_Normal/4_August_2008 4] [http://2008.igem.org/Beijing_Normal/5_August_2008 5] [http://2008.igem.org/Beijing_Normal/6_August_2008 6] [http://2008.igem.org/Beijing_Normal/7_August_2008 7] [http://2008.igem.org/Beijing_Normal/8_August_2008 8] [http://2008.igem.org/Beijing_Normal/9_August_2008 9] [http://2008.igem.org/Beijing_Normal/10_August_2008 10]
[http://2008.igem.org/Beijing_Normal/11_August_2008 11] [http://2008.igem.org/Beijing_Normal/12_August_2008 12] [http://2008.igem.org/Beijing_Normal/13_August_2008 13] [http://2008.igem.org/Beijing_Normal/14_August_2008 14] [http://2008.igem.org/Beijing_Normal/15_August_2008 15] [http://2008.igem.org/Beijing_Normal/16_August_2008 16] [http://2008.igem.org/Beijing_Normal/17_August_2008 17]
[http://2008.igem.org/Beijing_Normal/18_August_2008 18] [http://2008.igem.org/Beijing_Normal/19_August_2008 19] [http://2008.igem.org/Beijing_Normal/20_August_2008 20] [http://2008.igem.org/Beijing_Normal/21_August_2008 21] [http://2008.igem.org/Beijing_Normal/22_August_2008 22] [http://2008.igem.org/Beijing_Normal/23_August_2008 23] [http://2008.igem.org/Beijing_Normal/24_August_2008 24]
[http://2008.igem.org/Beijing_Normal/25_August_2008 25] [http://2008.igem.org/Beijing_Normal/26_August_2008 26] [http://2008.igem.org/Beijing_Normal/27_August_2008 27] [http://2008.igem.org/Beijing_Normal/28_August_2008 28] [http://2008.igem.org/Beijing_Normal/29_August_2008 29] [http://2008.igem.org/Beijing_Normal/30_August_2008 30] [http://2008.igem.org/Beijing_Normal/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/1_September_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/2_September_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/3_September_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/4_September_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/5_September_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/6_September_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/8_September_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/9_September_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/10_September_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/11_September_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/12_September_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_September_2008&action=edit 14]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_September_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_September_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_September_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/18_September_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/19_September_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/20_September_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/21_September_2008&action=edit 21]
[http://2008.igem.org/Beijing_Normal/22_September_2008 22] [http://2008.igem.org/Beijing_Normal/23_September_2008 23] [http://2008.igem.org/Beijing_Normal/24_September_2008 24] [http://2008.igem.org/Beijing_Normal/25_September_2008 25] [http://2008.igem.org/Beijing_Normal/26_September_2008 26] [http://2008.igem.org/Beijing_Normal/27_September_2008 27] [http://2008.igem.org/Beijing_Normal/28_September_2008 28]
[http://2008.igem.org/Beijing_Normal/29_September_2008 29] [http://2008.igem.org/Beijing_Normal/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/Beijing_Normal/1_October_2008 1] [http://2008.igem.org/Beijing_Normal/2_October_2008 2] [http://2008.igem.org/Beijing_Normal/3_October_2008 3] [http://2008.igem.org/Beijing_Normal/4_October_2008 4] [http://2008.igem.org/Beijing_Normal/5_October_2008 5]
[http://2008.igem.org/Beijing_Normal/6_October_2008 6] [http://2008.igem.org/Beijing_Normal/7_October_2008 7] [http://2008.igem.org/Beijing_Normal/8_October_2008 8] [http://2008.igem.org/Beijing_Normal/9_October_2008 9] [http://2008.igem.org/Beijing_Normal/10_October_2008 10] [http://2008.igem.org/Beijing_Normal/11_October_2008 11] [http://2008.igem.org/Beijing_Normal/12_October_2008 12]
[http://2008.igem.org/Beijing_Normal/13_October_2008 13] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/24_October_2008&action=edit 24] [http://2008.igem.org/Beijing_Normal/25_October_2008 25] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/31_October_2008&action=edit 31]
November
MTWTFSS
          [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/1_November_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/2_November_2008&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/3_November_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/4_November_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/5_November_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/6_November_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/7_November_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/8_November_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/9_November_2008&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/10_November_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/11_November_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/12_November_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/13_November_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/14_November_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/15_November_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/16_November_2008&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/17_November_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/18_November_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/19_November_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/20_November_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/21_November_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/22_November_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/23_November_2008&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Beijing_Normal/24_November_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/25_November_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/26_November_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/27_November_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/28_November_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/29_November_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Beijing_Normal/30_November_2008&action=edit 30]