Team:Bologna

From 2008.igem.org

(Difference between revisions)
Line 59: Line 59:
|titolo=[[Team:Bologna/Software|SOFTWARE]]
|titolo=[[Team:Bologna/Software|SOFTWARE]]
|contenuto=[[Image:cuscino.JPG|260px|right]]
|contenuto=[[Image:cuscino.JPG|260px|right]]
-
Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this[[Team:Bologna/Software|page]].
+
Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this [[Team:Bologna/Software|page]].
<br>
<br>
}}
}}

Revision as of 23:56, 29 October 2008

Logo1a.gifTestata dx.jpg
HOME PROJECT TEAM SOFTWARE MODELING WET LAB LAB-BOOK SUBMITTED PARTS BIOSAFETY AND PROTOCOLS


Logo Bologna


PROJECT
Nome Progetto

Our project aims to design a bacterial reprogrammable binary memory (EPROM) with genetically engineered E.coli. To engineer bacteria we designed a genetic Flip-Flop formed by an epigenetic memory sensitive to IPTG - for memory reset- and an UV-sensitive trigger to set the memory ON. We designed the Flip-Flop by model-based analysis. The core elements are two mutually regulated promoters. Each of them is composed of a constitutive promoter and an independent operator sequence. Thus, transcriptional strength and repressor binding affinity can be independently fixed. Since operator sites are still lacking in the Registry as standard parts, we cloned operator sequences for LacI, TetR, Lambda and LexA repressors and established an experimental procedure to characterize them. These parts allow the rational design of regulated promoters and we expect them to be a benefit in many Synthetic Biology applications. Click here for more information...



TEAM
TeamBo2.JPG

The leading idea behind our Ecoli.PROM team was to put in touch students with different curricula, ways to approach scientific problems and favour ideas exchange. These students, pushed by their common desire for new experiences, decided to participate in this exciting competition to discover a new research field and to expand their interest for synthetic biology. Responding to the call of Professor Silvio Cavalcanti, they constituted the Bologna iGEM team, finally composed of students from Biotechnology, Electronics Engineering and Biomedical Engineering faculties.
Go to the page!


MODELING
Circuito2.jpg

The aim of this work area is to determine the characteristics of the promoters that form the circuit. In this section are also collected a brief description of our model, all the equations that describe analytically our circuit, the most meaningful graphics and the results of simulations. Enjoy yourself!

SOFTWARE
Cuscino.JPG

Into this section you can find two software tools developed in our lab for this competition and that we would like to share with other Teams. One tool is a bacteria fluorescence image analyzer that segments the bacteria, counts their number, computes the size and the fluorescence intensity for each segmented bacterium. The second tool searches data in the Registry automatically to find parts by entry a part name or a string with short part description or a nucleotide sequence. You can download them from this page.


WET LAB
Collage2.JPG








To obtain regulated promoters we synthesized four libraries of operator sequences, respectively for LacI, TetR, cI and LexA repressor proteins. Here is how we designed the operator libraries, how we isolated single operators and try to clone them in the standard format and how we used operators to tune promoter activation in response to UV induction.So wet!







































Aknowledgements

Our Team is funded by:


  • [http://www.unibo.it/Portale/default.htm University of Bologna]


AlmaMaterStudiorum.jpg



  • [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]


Ser In Ar.jpg





Up