Team:Hawaii/Notebook/2008-10-15

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Things we did today

Wetlab work

Construction of secretion device (cont.)

Grace
  • Transformed DH5α
  • Used 5 μl of n/p + rgt ligation reaction
  • Used 1 μl of J33207/pRL1383a ligation reaction
  • Plated 25 μl, 50 μl, 75 μl, and 100 μl plates in hopes of isolating single colonies

Ligation

Margaret
  • lac promoter/rbs(34) (E,S) + rep2 (X,P) + pSB1A3 (de-phosphorylated, E,P)
  • lac promoter/rbs(30)rep/oriV(6) (E,S) + lac promoter/rbs(34)aada/oriT(A) (X,P) +pSB1A3 (de-phosphorylated, E,P)
  • lac promoter/rbs(30)rep/oriV(6) + lac promoter/rbs(34)aada/oriT (A)
  • lac promoter/rbs(30)rep/oriV (6) (E,S) + lac promoter/rbs(34)aada/oriT(B) (X,P) +pSB1A3(de-phosphorylated, E,P)
  • oriV (E,S) + oriT (X,P) + pSB1A7 (E,P)
  • pSB1A7 self (did not de-phosphorylate) (E,P)


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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