Team:Hawaii/Notebook/2008-10-16

From 2008.igem.org

Revision as of 03:25, 19 October 2008 by MargaretRuzicka (Talk | contribs)
Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour.
Construct Colonies
nir + rbs+GFP+tt in BBpRL1383a-1 2
plac + rbs+GFP+tt in BBpRL1383a-1 0
J33207 in pRL1383a (all plates) lawn
  • Colony PCR of transformants
  • None of the colonies tested positive for their desired inserts

Transformation

Margaret
Construct Colonies
lac promoter/rbs(34)+ rep + pSB1A2 0
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3 113
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) 141
lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3 150
oriV (6) + oriT + pSB1A7 200
pSB1A7 self (did not de-phosphorylate) 50
DH5-a with RP4 3
DB3.1 with RP4 0
(+) pUC18 lawn
(-) no plasmid 0


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]