Team:The University of Alberta/Butanerd
From 2008.igem.org
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<h4>'''The Summer Summary'''</h4> | <h4>'''The Summer Summary'''</h4> | ||
- | + | :The summer began with working on the butanol project as a training exercise to get the team familiarized with lab procedures. The team worked on ligating individual butanol inserts into the J61003 and I0500 vectors. Furthermore, protein expressions were to be done on these plasmids once the ligations were successfully completed. The ligations were a success by the beginning of July. | |
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+ | :The team proceeded to show protein expression of the individual butanol inserts. The Western blots were successfully completed by the end of July. | ||
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+ | :Finally, with the eagerness of the team to complete last year's project, the completion of the full butanol operon was adopted as a side project. The full operon (from insert 21 to insert 25) was successfully assembled in PSB1A3 by mid-October! | ||
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<h4>'''Back to [https://2008.igem.org/Team:The_University_of_Alberta/Project the projects page].'''</h4> | <h4>'''Back to [https://2008.igem.org/Team:The_University_of_Alberta/Project the projects page].'''</h4> |
Revision as of 17:13, 28 October 2008
Contents |
The Butanerd Project Continues!
For background information on the butanol project, please view the iGEM 2007: Alberta team page.
Inserts and Promoters/Vectors Used
- RBS + B-hydroxy butyryl coA dehydrogenase (AKA. 21)
- RBS + Enoyl-coa hydratase (AKA. 22)
- RBS + Butyryl coa Dehydrogenase (AKA. 23)
- RBS + Butyraldehyde dehydrogenase (AKA. 24)
- RBS + Butanol dehydrogenase (AKA. 25)
- All the parts submitted by last year's team can be found here.
The Summer Summary
- The summer began with working on the butanol project as a training exercise to get the team familiarized with lab procedures. The team worked on ligating individual butanol inserts into the J61003 and I0500 vectors. Furthermore, protein expressions were to be done on these plasmids once the ligations were successfully completed. The ligations were a success by the beginning of July.
- The team proceeded to show protein expression of the individual butanol inserts. The Western blots were successfully completed by the end of July.
- Finally, with the eagerness of the team to complete last year's project, the completion of the full butanol operon was adopted as a side project. The full operon (from insert 21 to insert 25) was successfully assembled in PSB1A3 by mid-October!