Team:UCSF/Parts

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'''AarI TOPO clones''' (digestion of these plasmids with AarI will release a fragment for AarI cloning).
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'''Project-relevant AarI TOPO clones''' (digestion of these plasmids with AarI will release a fragment for AarI cloning).
GFP BD
GFP BD
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Sas2 BD
Sas2 BD
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'''AarI acceptor vectors (empty)'''
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''These accept AarI digested parts, and include a promoter/terminator.''
'''AarI Shuttle Vector'''
'''AarI Shuttle Vector'''
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'''AarI Acceptor Vectors'''
 
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pRS315, Adh1P, Adh1t: CEN ARS vector for yeast expression (LEU2 marker), with Adh1 promoter and terminator.
 

Revision as of 04:36, 29 October 2008

Project-relevant AarI TOPO clones (digestion of these plasmids with AarI will release a fragment for AarI cloning).

GFP BD

Sir4

Sir2 AB

Sir2 BD

mCherry AB

mCherry BD

LexA (1-87) AB

LexA (1-87) BD

Pif3 AB

PhyB AB

Sas2 AB

Sas2 BD

AarI acceptor vectors (empty)

These accept AarI digested parts, and include a promoter/terminator.

AarI Shuttle Vector

So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.

  • AarI--Biobrick Shuttle AB
  • AarI--Biobrick Shuttle BD


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Esa1 AB




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