Team:UCSF/Parts

From 2008.igem.org

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'''Project-relevant AarI TOPO clones''' (digestion of these plasmids with AarI will release a fragment for AarI cloning).
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For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1
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'''Project-relevant AarI TOPO clones'''  
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''digestion of these plasmids with AarI will release a fragment for AarI cloning.''
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*LexA (1-87) AB
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*Sir2 BD
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*GFP AD
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GFP BD
GFP BD
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Sir2 AB
Sir2 AB
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Sir2 BD
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mCherry AB
mCherry AB
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mCherry BD
mCherry BD
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LexA (1-87) AB
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LexA (1-87) BD
LexA (1-87) BD

Revision as of 04:40, 29 October 2008

For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1


Project-relevant AarI TOPO clones

digestion of these plasmids with AarI will release a fragment for AarI cloning.

  • LexA (1-87) AB
  • Sir2 BD
  • GFP AD


GFP BD

Sir4

Sir2 AB


mCherry AB

mCherry BD


LexA (1-87) BD

Pif3 AB

PhyB AB

Sas2 AB

Sas2 BD

AarI acceptor vectors (empty)

These accept AarI digested parts, and include a promoter/terminator.

AarI Shuttle Vector

So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.

  • AarI--Biobrick Shuttle AB
  • AarI--Biobrick Shuttle BD


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Esa1 AB




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