Team:UCSF/Parts

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AarI TOPO clones (digestion of these plasmids with AarI will release a fragment for AarI cloning).

GFP BD

Sir4

Sir2 AB

Sir2 BD

mCherry AB

mCherry BD

LexA (1-87) AB

LexA (1-87) BD

Pif3 AB

PhyB AB

Sas2 AB

Sas2 BD

AarI Shuttle Vector So you don't want to use AarI cloning? We've provided a shuttle vector that you can subclone an AarI AB or BD part into, then cut it back out with biobrick sites.

AarI--Biobrick Shuttle AB

AarI--Biobrick Shuttle BD


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Esa1 AB


AarI Acceptor Vectors

pRS315, Adh1P, Adh1t: CEN ARS vector for yeast expression (LEU2 marker), with Adh1 promoter and terminator.



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