Team:University of Lethbridge/Modeling

From 2008.igem.org

Revision as of 04:18, 27 October 2008 by Selina.dobing (Talk | contribs)
UofLteamlogo.jpg

[http://2008.igem.org/wiki/index.php?title=Team:University_of_Lethbridge&Home UofLhomebutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Team UofLteambutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Project UofLprojectbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Parts UofLpartsbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Modeling UofLmodelingbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Notebook UofLnotebookbutton.jpg]

UofLteamlogo.jpg

Our Project

For our project, we decided to create a synthetic bacterium which could move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium.

We used the modeling program BCT (ref) to determine the level of CheZ gene expression necessary to produce dephosphorylated CheY and directed movement. Increasing intracellular concentrations of CheZ leads to increased CheY in the unphosphorylated state (Figure 1a)...



Igem chez figure1.JPG Igem chez figure2.JPG
Figure 1(a) Figure 1(b)


Igem chez combo.jpg

Alternative Figure 1 -need to add 2nd axes title


UofLteamlogo.jpg

[http://2008.igem.org/wiki/index.php?title=Team:University_of_Lethbridge&Home UofLhomebutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Team UofLteambutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Project UofLprojectbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Parts UofLpartsbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Modeling UofLmodelingbutton.jpg]

[http://2008.igem.org/Team:University_of_Lethbridge/Notebook UofLnotebookbutton.jpg]

UofLteamlogo.jpg


</div>