Team:University of Lethbridge/Notebook/Project1September

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===September 10, 2008===
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====Selina, Christa, Munima====
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Created 10 mL sterile 100 mM theophylline solution. Aliquotted into microfuge tubes and placed in cabinet in teaching lab.
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Note: higher solubility in EtOH (see Merck index) but will only reach 100 mM in a super-saturated H20 solution (heat up to 50C directly before use).
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===September 11, 2008===
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====Christa, Selina, Munima====
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Made motility media tubes (~20 blank, ~12 with 1 mM theophylline, ~10 with 0.25 mM theophylline, 1 with 50 mM theophylline). Attempts to make 'layered' motility media stab tubes was a complete disaster.
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Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!

Revision as of 15:21, 12 September 2008

Back to The University of Lethbridge Main Notebook

Contents

September 10, 2008

Selina, Christa, Munima

Created 10 mL sterile 100 mM theophylline solution. Aliquotted into microfuge tubes and placed in cabinet in teaching lab.

Note: higher solubility in EtOH (see Merck index) but will only reach 100 mM in a super-saturated H20 solution (heat up to 50C directly before use).


September 11, 2008

Christa, Selina, Munima

Made motility media tubes (~20 blank, ~12 with 1 mM theophylline, ~10 with 0.25 mM theophylline, 1 with 50 mM theophylline). Attempts to make 'layered' motility media stab tubes was a complete disaster.

Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!

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