Team:University of Ottawa/25 July 2008

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'''Dan'''
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:'''Miniprep'''
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::<li> Minipreped the 6 colonies, obatined good concentrations however gel confirmation revealed that transformation was unsuccessful.
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:'''Gel Extraction'''
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::<li> Performed gel extraction of T123 amplification product from July 24
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::<li> To much DNA was inserted into each well, will have to do PCR again
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:'''PCR of 0A and 0B'''
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::<li> PCR of constructs 0A and 0B was performed, producing lots of DNA.
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Revision as of 14:46, 12 August 2008

Untitled Document

 

 

'''Dan''' :'''Miniprep''' ::
  • Minipreped the 6 colonies, obatined good concentrations however gel confirmation revealed that transformation was unsuccessful. :'''Gel Extraction''' ::
  • Performed gel extraction of T123 amplification product from July 24 ::
  • To much DNA was inserted into each well, will have to do PCR again :'''PCR of 0A and 0B''' ::
  • PCR of constructs 0A and 0B was performed, producing lots of DNA.

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